Journal article
Self-assembling asymmetric peptide-dendrimer micelles – a platform for effective and versatile in vitro nucleic acid delivery
Scientific Reports, Vol.8(1)
2018
Abstract
Despite advancements in the development of high generation cationic-dendrimer systems for delivery of nucleic acid-based therapeutics, commercially available chemical agents suffer from major drawbacks such as cytotoxicity while being laborious and costly to synthesize. To overcome the aforementioned limitations, low-generation cationic peptide asymmetric dendrimers with side arm lipid (cholic and decanoic acid) conjugation were designed, synthesized and systematically screened for their ability to self-assemble into micelles using dynamic light scattering. Cytotoxicity profiling revealed that our entire asymmetric peptide dendrimer library when trialled alone, or as asymmetric dendrimer micelle-nucleic acid complexes, were non-cytotoxic across a broad concentration range. Further, the delivery efficiency of asymmetric peptide dendrimers in H-4-II-E (rat hepatoma), H2K (mdx mouse myoblast), and DAOY (human medulloblastoma) cells demonstrated that cholic acid-conjugated asymmetric dendrimers possess far superior delivery efficiency when compared to the commercial standards, Lipofectamine 2000 or Lipofectin®.
Details
- Title
- Self-assembling asymmetric peptide-dendrimer micelles – a platform for effective and versatile in vitro nucleic acid delivery
- Authors/Creators
- G.R. Kokil (Author/Creator) - The University of QueenslandR.N. Veedu (Author/Creator) - Murdoch UniversityB.T. Le (Author/Creator) - Murdoch UniversityG.A. Ramm (Author/Creator) - QIMR Berghofer Medical Research InstituteH.S. Parekh (Author/Creator) - The University of Queensland
- Publication Details
- Scientific Reports, Vol.8(1)
- Publisher
- Springer Nature
- Identifiers
- 991005543393707891
- Copyright
- © 2018 The Author(s)
- Murdoch Affiliation
- Centre for Comparative Genomics
- Language
- English
- Resource Type
- Journal article
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- Collaboration types
- Domestic collaboration
- Citation topics
- 2 Chemistry
- 2.53 Polymers & Macromolecules
- 2.53.70 Nanocarrier Drug Delivery
- Web Of Science research areas
- Pharmacology & Pharmacy
- ESI research areas
- Pharmacology & Toxicology