Journal article
Semi-quantitative analysis of cannabinoids in hemp (Cannabis sativa L.) using gas chromatography coupled to mass spectrometry
Journal of Cannabis Research, Vol.4(1), Art. 51
2022
Abstract
Background
Hemp (Cannabis sativa L.) is a producer of cannabinoids. These organic compounds are of increasing interest due to their potential applications in the medicinal field. Advances in analytical methods of identifying and quantifying these molecules are needed.
Method
This study describes a new method of cannabinoid separation from plant material using gas chromatography-mass spectrometry (GC-MS) as the analytical tool to detect low abundance cannabinoids that will likely have implications for future therapeutical treatments. A novel approach was adopted to separate trichomes from plant material to analyse cannabinoids of low abundance not observed in raw plant extract. Required plant sample used for analysis was greatly reduced compared to other methods. Derivatisation method was simplified and deconvolution software was utilised to recognise unknown cannabinoid compounds of low abundance.
Results
The method produces well-separated spectra and allows the detection of major and minor cannabinoids. Ten cannabinoids that had available standards could be identified and quantified and numerous unidentified cannabinoids or pathway intermediates based on GC-MS spectra similarities could be extracted and analysed simultaneously with this method.
Conclusions
This is a rapid novel extraction and analytical method from plant material that can identify major and minor cannabinoids using a simple technique. The method will be of use to future researchers seeking to study the multitude of cannabinoids whose values are currently not understood.
Details
- Title
- Semi-quantitative analysis of cannabinoids in hemp (Cannabis sativa L.) using gas chromatography coupled to mass spectrometry
- Authors/Creators
- L. De Prato (Author/Creator) - Murdoch UniversityM. Timmins (Author/Creator)O. Ansari (Author/Creator)K.X. Ruthrof (Author/Creator)G.E.St.J. Hardy (Author/Creator)J. Howieson (Author/Creator)G. O’Hara (Author/Creator)
- Publication Details
- Journal of Cannabis Research, Vol.4(1), Art. 51
- Publisher
- BioMed Central Ltd as part of Springer Nature
- Identifiers
- 991005540919907891
- Copyright
- © 2022 The Authors.
- Murdoch Affiliation
- Food Futures Institute
- Language
- English
- Resource Type
- Journal article
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