Sequence analysis of mRNA transcripts encoding Jembrana disease virus Tat-1 in vivo

Surachmi Setiyaningsih; M. Desport; M.E. Stewart; N. Hartaningsih; G.E. Wilcox

doi:10.1016/j.virusres.2007.11.004

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Sequence analysis of mRNA transcripts encoding Jembrana disease virus Tat-1 in vivo

Journal article

Peer reviewed

Sequence analysis of mRNA transcripts encoding Jembrana disease virus Tat-1 in vivo

Surachmi Setiyaningsih, M. Desport, M.E. Stewart, N. Hartaningsih and G.E. Wilcox

Virus Research, Vol.132(1-2), pp.220-225

2008

DOI: https://doi.org/10.1016/j.virusres.2007.11.004

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Abstract

Jembrana disease virus (JDV) is a lentivirus which replicates to very high titres in vivo and its Tat-1 protein has been shown to be a potent transactivator in vitro. Analysis of tat mRNA transcripts produced early in infection studies identified four predominant species which were generated by multiple splicing events. The use of a splice donor downstream of tat-1 was common indicating that a Tat-1 protein of 97 amino acids is expressed during the acute phase of Jembrana disease. The presence of an in-frame stop codon between tat-1 and tat-2 was identified in transcripts from three different strains of JDV confirming that expression of a single exon Tat-1 correlates with high viral titres in vivo. Sequence conservation in the regions of tat-1 that are critical for RNA binding and transcription activation in three different virus strains was high and the tat-2 sequences were completely conserved.

Details

Title: Sequence analysis of mRNA transcripts encoding Jembrana disease virus Tat-1 in vivo
Authors/Creators: Surachmi Setiyaningsih (Author/Creator) - Murdoch University
M. Desport (Author/Creator) - Murdoch University
M.E. Stewart (Author/Creator) - Murdoch University
N. Hartaningsih (Author/Creator) - Disease Investigation Centre, Region VI, PO Box 3322, Indonesia
G.E. Wilcox (Author/Creator) - Murdoch University
Publication Details: Virus Research, Vol.132(1-2), pp.220-225
Publisher: Elsevier BV
Identifiers: 991005541753107891
Copyright: 2007 Elsevier BV
Murdoch Affiliation: School of Veterinary and Biomedical Sciences
Language: English
Resource Type: Journal article

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Citation topics: 1 Clinical & Life Sciences; 1.66 HIV; 1.66.46 HIV Pathogenesis
Web Of Science research areas: Virology
ESI research areas: Microbiology