Sequence differences in the diagnostic target region of the oocyst wall protein gene of Cryptosporidium parasites

L. Xiao; J. Limor; U.M. Morgan; I. Sulaiman; R.C.A. Thompson; A.A. Lal

doi:10.1128/AEM.66.12.5499-5502.2000

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Sequence differences in the diagnostic target region of the oocyst wall protein gene of Cryptosporidium parasites

Journal article

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Peer reviewed

Sequence differences in the diagnostic target region of the oocyst wall protein gene of Cryptosporidium parasites

L. Xiao, J. Limor, U.M. Morgan, I. Sulaiman, R.C.A. Thompson and A.A. Lal

Applied and Environmental Microbiology, Vol.66(12), pp.5499-5502

2000

DOI: https://doi.org/10.1128/AEM.66.12.5499-5502.2000

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Abstract

Nucleotide sequences of the Cryptosporidium oocyst wall protein (COWP) gene were obtained from Various Cryptosporidium spp. (C. wrairi, C. felis, C. meleagridis, C. baileyi, C. andersoni, C. muris, and C. serpentis) and C. parvum genotypes (human, bovine, monkey, marsupial, ferret, mouse, pig, and dog). Significant diversity was observed among species and genotypes in the primer and target regions of a popular diagnostic PCR. These results provide useful information for COWP-based molecular differentiation of Cryptosporidium spp. and genotypes.

Details

Title: Sequence differences in the diagnostic target region of the oocyst wall protein gene of Cryptosporidium parasites
Authors/Creators: L. Xiao (Author/Creator) - United States Department of Health and Human Services
J. Limor (Author/Creator) - United States Department of Health and Human Services
U.M. Morgan (Author/Creator) - Murdoch University
I. Sulaiman (Author/Creator) - United States Department of Health and Human Services
R.C.A. Thompson (Author/Creator) - Murdoch University
A.A. Lal (Author/Creator) - United States Department of Health and Human Services
Publication Details: Applied and Environmental Microbiology, Vol.66(12), pp.5499-5502
Publisher: American Society for Microbiology
Identifiers: 991005542787807891
Copyright: © 2000 American Society for Microbiology
Murdoch Affiliation: State Agricultural Biotechnology Centre; School of Veterinary and Biomedical Sciences
Language: English
Resource Type: Journal article

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Collaboration types: Domestic collaboration; International collaboration
Citation topics: 1 Clinical & Life Sciences; 1.246 Diarrheal Diseases; 1.246.985 Cryptosporidium
Web Of Science research areas: Biotechnology & Applied Microbiology; Microbiology
ESI research areas: Biology & Biochemistry