Journal article
Sequence variation and immunologic cross-reactivity among Babesia bovis merozoite surface antigen 1 proteins from vaccine strains and vaccine breakthrough isolates
Infection and Immunity, Vol.73(9), pp.5388-5394
2005
Abstract
The Babesia bovis merozoite surface antigen 1 (MSA-1) is an immunodominant membrane glycoprotein that is the target of invasion-blocking antibodies. While antigenic variation has been demonstrated in MSA-1 among strains from distinct geographical areas, the extent of sequence variation within a region where it is endemic and the effect of variation on immunologic cross-reactivity have not been assessed. In this study, sequencing of MSA-1 from two Australian B. bovis vaccine strains and 14 breakthrough isolates from vaccinated animals demonstrated low sequence identity in the extracellular region of the molecule, ranging from 19.8 to 46.7% between the T vaccine strain and eight T vaccine breakthrough isolates, and from 18.7 to 99% between the K vaccine strain and six K vaccine breakthrough isolates. Although MSA-1 amino acid sequence varied substantially among strains, overall predicted regions of hydrophilicity and hydrophobicity in the extracellular domain were conserved in all strains examined, suggesting a conserved functional role for MSA-1 despite sequence polymorphism. Importantly, the antigenic variation created by sequence differences resulted in a lack of immunologic cross-reactivity among outbreak strains using sera from animals infected with the B. bovis vaccine strains. Additionally, sera from cattle hyperinfected with the Mexico strain of B. bovis and shown to be clinically immune did not cross-react with MSA-1 from any other isolate tested. The results indicate that isolates of B. bovis capable of evading vaccine-induced immunity contain an msa-1 gene that is significantly different from the msa-1 of the vaccine strain, and that the difference can result in a complete lack of cross-reactivity between MSA-1 from vaccine and breakthrough strains in immunized animals.
Details
- Title
- Sequence variation and immunologic cross-reactivity among Babesia bovis merozoite surface antigen 1 proteins from vaccine strains and vaccine breakthrough isolates
- Authors/Creators
- T. LeRoith (Author/Creator) - Washington State UniversityK.A. Brayton (Author/Creator) - Washington State UniversityJ.B. Molloy (Author/Creator) - Emerging Technologies/Delivery, Queensland Department of Primary Industries and Fisheries, Moorooka 4105, Queensland, AustraliaR.E. Bock (Author/Creator) - Tick Fever Centre, Biosecurity, Queensland Department of Primary Industries and Fisheries, Wacol, Queensland, AustraliaS.A. Hines (Author/Creator) - Washington State UniversityA.E. Lew (Author/Creator) - Emerging Technologies/Delivery, Queensland Department of Primary Industries and Fisheries, Moorooka 4105, Queensland, AustraliaT.F. McElwain (Author/Creator) - Washington State University
- Publication Details
- Infection and Immunity, Vol.73(9), pp.5388-5394
- Publisher
- American Society for Microbiology
- Identifiers
- 991005542617807891
- Copyright
- 2005 American Society for Microbiology
- Murdoch Affiliation
- Murdoch University
- Language
- English
- Resource Type
- Journal article
UN Sustainable Development Goals (SDGs)
This output has contributed to the advancement of the following goals:
Source: InCites
Metrics
140 File views/ downloads
58 Record Views
InCites Highlights
These are selected metrics from InCites Benchmarking & Analytics tool, related to this output
- Collaboration types
- Domestic collaboration
- International collaboration
- Citation topics
- 1 Clinical & Life Sciences
- 1.258 Zoonotic Diseases
- 1.258.227 Tick-borne Pathogens
- Web Of Science research areas
- Immunology
- Infectious Diseases
- ESI research areas
- Immunology