Journal article
Sequencing analysis of genetic loci for resistance for late leaf spot and rust in peanut (Arachis hypogaea L.)
Frontiers in Plant Science, Vol.9, Art. 01727
2018
Abstract
The aim of this study was to identify candidate resistance genes for late leaf spot (LLS) and rust diseases in peanut (Arachis hypogaea L.). We used a double-digest restriction-site associated DNA sequencing (ddRAD-Seq) technique based on next-generation sequencing (NGS) for genotyping analysis across the recombinant inbred lines (RILs) derived from a cross between a susceptible line, TAG 24, and a resistant line, GPBD 4. A total of 171 SNPs from the ddRAD-Seq together with 282 markers published in the previous studies were mapped on a genetic map covering 1510.1 cM. Subsequent quantitative trait locus (QTL) analysis revealed major genetic loci for LLS and rust resistance on chromosomes A02 and A03, respectively. Heterogeneous inbred family-derived near isogenic lines and the pedigree of the resistant gene donor, A. cardenasii Krapov. & W.C. Greg., including the resistant derivatives of ICGV 86855 and VG 9514 as well as GPBD 4, were employed for whole-genome resequencing analysis. The results indicated the QTL candidates for LLS and rust resistance were located in 1.4- and 2.7-Mb genome regions on A02 and A03, respectively. In these regions, four and six resistance-related genes with deleterious mutations were selected as candidates for LLS and rust resistance, respectively. These delimited genomic regions may be beneficial in breeding programs aimed at improving disease resistance and enhancing peanut productivity.
Details
- Title
- Sequencing analysis of genetic loci for resistance for late leaf spot and rust in peanut (Arachis hypogaea L.)
- Authors/Creators
- K. Shirasawa (Author/Creator) - Kazusa DNA Research InstituteR.S. Bhat (Author/Creator) - University of Agricultural Sciences, DharwadY.P. Khedikar (Author/Creator) - University of Agricultural Sciences, DharwadV. Sujay (Author/Creator) - University of Agricultural Sciences, DharwadR.M. Kolekar (Author/Creator) - University of Agricultural Sciences, DharwadS.B. Yeri (Author/Creator) - University of Agricultural Sciences, DharwadM. Sukruth (Author/Creator) - University of Agricultural Sciences, DharwadS. Cholin (Author/Creator) - University of Agricultural Sciences, DharwadB. Asha (Author/Creator) - University of Agricultural Sciences, DharwadM.K. Pandey (Author/Creator) - International Crops Research Institute for the Semi-Arid TropicsR.K. Varshney (Author/Creator) - International Crops Research Institute for the Semi-Arid TropicsM.V.C. Gowda (Author/Creator) - University of Agricultural Sciences, Dharwad
- Publication Details
- Frontiers in Plant Science, Vol.9, Art. 01727
- Publisher
- Frontiers
- Identifiers
- 991005541672507891
- Copyright
- © 2018 The Authors.
- Murdoch Affiliation
- Murdoch University
- Language
- English
- Resource Type
- Journal article
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- Collaboration types
- Domestic collaboration
- International collaboration
- Citation topics
- 3 Agriculture, Environment & Ecology
- 3.4 Crop Science
- 3.4.96 QTL
- Web Of Science research areas
- Plant Sciences
- ESI research areas
- Plant & Animal Science