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Journal article
Sequencing of the viral UL111a gene directly from clinical specimens reveals variants of HCMV-encoded IL-10 that are associated with altered immune responses to HCMV
International Journal of Molecular Sciences, Vol.23(9), Article 4644
2022
Abstract
Human cytomegalovirus (HCMV) is a beta-herpesvirus carried by ~80% of adults worldwide. Acute infections are often asymptomatic in healthy individuals but generate diverse syndromes in neonates, renal transplant recipients (RTR), and people with HIV (PWH). The HCMV gene UL111a encodes a homolog of human interleukin-10 (IL-10) that interacts with the human IL-10 receptor. Deep sequencing technologies were used to sequence UL111a directly from 59 clinical samples from Indonesian PWH and Australian RTR, healthy adults, and neonates. Overall, 93% of samples contained more than one variant of HCMV, as defined by at least one nonsynonymous variation. Carriage of these variants differed between neonates and adults, Australians and Indonesians, and between saliva and blood leukocytes. The variant alleles of N41D and S71Y occurred together in Australian RTR and were associated with higher T-cell responses to HCMV pp65. The variant P122S was associated with lower levels of antibodies reactive with a lysate of HCMV-infected fibroblasts. L174F was associated with increased levels of antibodies reactive with HCMV lysate, immediate-early 1 (IE-1), and glycoprotein B (gB) in Australian RTR and Indonesians PWH, suggesting a higher viral burden. We conclude that variants of UL111a are common in all populations and may influence systemic responses to HCMV.
Details
- Title
- Sequencing of the viral UL111a gene directly from clinical specimens reveals variants of HCMV-encoded IL-10 that are associated with altered immune responses to HCMV
- Authors/Creators
- S. Waters (Author/Creator) - Curtin UniversityS. Lee (Author/Creator) - Curtin UniversityI. Ariyanto (Author/Creator) - University of IndonesiaN. Kresoje (Author/Creator) - The University of Western AustraliaS. Leary (Author/Creator) - Murdoch UniversityK. Munyard (Author/Creator) - Curtin UniversityS. Gaudieri (Author/Creator) - Murdoch UniversityA. Irish (Author/Creator)A.D. Keil (Author/Creator) - Pathwest Laboratory MedicineR.J.N. Allcock (Author/Creator) - The University of Western AustraliaP. Price (Author/Creator) - Fiona Stanley Hospital
- Publication Details
- International Journal of Molecular Sciences, Vol.23(9), Article 4644
- Publisher
- MDPI
- Identifiers
- 991005540492907891
- Copyright
- © 2022 by the authors
- Murdoch Affiliation
- Institute for Immunology and Infectious Diseases
- Language
- English
- Resource Type
- Journal article
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- Collaboration types
- Domestic collaboration
- International collaboration
- Citation topics
- 1 Clinical & Life Sciences
- 1.161 Virology - Identification & Sequencing
- 1.161.711 Cytomegalovirus Infections
- Web Of Science research areas
- Biochemistry & Molecular Biology
- Chemistry, Multidisciplinary
- ESI research areas
- Chemistry