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Serological evidence of an Australian bovine lentivirus
Journal article   Peer reviewed

Serological evidence of an Australian bovine lentivirus

E.J. Burkala, T.M. Ellis, V. Voigt and G.E. Wilcox
Veterinary Microbiology, Vol.68(1-2), pp.171-177
1999
DOI: https://doi.org/10.1016/S0378-1135(99)00073-5
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Abstract

Recombinant 26kDa capsid (CA) proteins of bovine lentiviruses, bovine immunodeficiency virus (BIV) and Jembrana disease virus (JDV), were expressed in Escherichia coli and utilised as antigens for an enzyme-linked immunosorbent assay (ELISA) and a western immunoblot (WIB) procedure for the detection of antibody in dairy cattle in Western Australia. A total of 690 serum samples, 30 from each of 23 farms, were tested by ELISA with a JDV CA protein antigen, and antibody was detected in 3.8% (p<0.05) of the sera. Nine sera from each farm were also tested by WIB with JDV CA protein antigens and antibody was detected in 15.9% of these samples. All ELISA-positive results were also WIB-positive, and all sera antibody-positive by WIB with JDV CA protein antigens were also antibody-positive by the WIB using recombinant BIV CA antigens. This study showed that recombinant protein antigens can be used for serological tests to detect bovine lentivirus infection in Australia.

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Source: InCites

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18 Times Cited - Web of Science

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Citation topics
1 Clinical & Life Sciences
1.66 HIV
1.66.46 HIV Pathogenesis
Web Of Science research areas
Microbiology
Veterinary Sciences
ESI research areas
Plant & Animal Science
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