Journal article
Serum Amyloid Alpha Is Downregulated in Peripheral Tissues of Parkinson’s Disease Patients
Frontiers in Neuroscience, Vol.13, Article number 13
2019
Abstract
We report the changed levels of serum amyloid alpha, an immunologically active protein, in Parkinson’s disease (PD) patients’ peripheral tissues. We have previously shown that Saa-1 and -2 (serum amyloid alpha-1,-2, genes) were among the top downregulated genes in PD patients’ skin, using whole-genome RNA sequencing. In the current study, we characterized the gene and protein expression profiles of skin and blood samples from patients with confirmed PD diagnosis and age/sex matched controls. qRT-PCR analysis of PD skin demonstrated downregulation of Saa-1 and -2 genes in PD patients. However, the lowered amount of protein could not be visualized using immunohistochemistry, due to low quantity of SAA (Serum Amyloid Alpha, protein) in skin. Saa-1 and -2 expression levels in whole blood were below detection threshold based on RNA sequencing, however significantly lowered protein levels of SAA1/2 in PD patients’ serum were shown with ELISA, implying that SAA is secreted into the blood. These results show that SAA is differentially expressed in the peripheral tissues of PD patients.
Details
- Title
- Serum Amyloid Alpha Is Downregulated in Peripheral Tissues of Parkinson’s Disease Patients
- Authors/Creators
- L. Kurvits (Author/Creator)E. Reimann (Author/Creator)L. Kadastik-Eerme (Author/Creator)L. Truu (Author/Creator)K. Kingo (Author/Creator)T. Erm (Author/Creator)S. Kõks (Author/Creator)P. Taba (Author/Creator)A. Planken (Author/Creator)
- Publication Details
- Frontiers in Neuroscience, Vol.13, Article number 13
- Publisher
- Frontiers
- Identifiers
- 991005545095707891
- Copyright
- © 2019 Kurvits, Reimann, Kadastik-Eerme, Truu, Kingo, Erm, Kõks, Taba and Planken
- Murdoch Affiliation
- Centre for Comparative Genomics
- Language
- English
- Resource Type
- Journal article
UN Sustainable Development Goals (SDGs)
This output has contributed to the advancement of the following goals:
Metrics
74 File views/ downloads
40 Record Views
InCites Highlights
These are selected metrics from InCites Benchmarking & Analytics tool, related to this output
- Collaboration types
- Domestic collaboration
- International collaboration
- Citation topics
- 1 Clinical & Life Sciences
- 1.52 Neurodegenerative Diseases
- 1.52.67 Parkinson's Disease
- Web Of Science research areas
- Neurosciences
- ESI research areas
- Neuroscience & Behavior