Journal article
Serum paraoxonase 1 activity in cats: Analytical validation, reference intervals, and correlation with serum amyloid A and alpha-1-acid glycoprotein
Journal of Veterinary Diagnostic Investigation, Vol.32(6), pp.844-855
2020
Abstract
Paraoxonase 1 (PON1) is an inflammation marker associated with lipid oxidation and is used as a diagnostic marker in people. There is no information about the suitable substrate and analytic performance in cats, or its biological behavior compared with other inflammation markers. Our aims were to validate a paraoxon-based method to measure PON1 activity in feline serum, to assess stability of PON1 under different storage conditions and the impact of interfering elements, to determine a reference interval (RI) for healthy cats, and to correlate PON1 activity with 2 major acute-phase proteins. Intra- and inter-assay precision, accuracy, and RI were assessed using fresh serum. The same specimens were stored at room temperature, refrigerated, or frozen, and retested at defined intervals. Hemolysis, lipemia, and icterus were simulated to study interferences. PON1 results were compared to serum amyloid A (SAA) and alpha-1-acid glycoprotein (AGP) results. Analytical validation yielded precise and accurate results. PON1 activity is stable for up to 24 h at room temperature and up to 48 h at 4°C. Freezing at −20°C results in an increase after 72 h, with return to baseline values after 1 wk, that again increases after 6 mo. Only hyperlipemia interfered with PON1 activity. The RI based on 71 healthy cats was 58–154 U/L. PON1 activity was negatively correlated with AGP, but not with SAA. Serum PON1 activity can be measured accurately in cats, and it acts as a negative acute-phase protein.
Details
- Title
- Serum paraoxonase 1 activity in cats: Analytical validation, reference intervals, and correlation with serum amyloid A and alpha-1-acid glycoprotein
- Authors/Creators
- G. Rossi (Author/Creator) - Murdoch UniversityS. Meazzi (Author/Creator)A. Giordano (Author/Creator) - University of MilanS. Paltrinieri (Author/Creator) - University of Milan
- Publication Details
- Journal of Veterinary Diagnostic Investigation, Vol.32(6), pp.844-855
- Publisher
- American Association of Veterinary Laboratory Diagnosticians
- Identifiers
- 991005541299507891
- Copyright
- © 2020 by American Association of Veterinary Laboratory Diagnosticians
- Murdoch Affiliation
- School of Veterinary and Life Sciences
- Language
- English
- Resource Type
- Journal article
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