Serum zonulin measured by enzyme-linked immunosorbent assay may not be a reliable marker of small intestinal permeability in healthy adults

O. Tatucu-Babet; A. Forsyth; E. Owen; D. Navarro-Perez; J. Radcliffe; D. Benheim; H. Mendis; M. Jois; C. Itsiopoulos; A. Tierney

doi:10.1016/j.nutres.2020.05.003

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Serum zonulin measured by enzyme-linked immunosorbent assay may not be a reliable marker of small intestinal permeability in healthy adults

Journal article

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Serum zonulin measured by enzyme-linked immunosorbent assay may not be a reliable marker of small intestinal permeability in healthy adults

O. Tatucu-Babet, A. Forsyth, E. Owen, D. Navarro-Perez, J. Radcliffe, D. Benheim, H. Mendis, M. Jois, C. Itsiopoulos and A. Tierney

Nutrition Research, Vol.78, pp.82-92

2020

DOI: https://doi.org/10.1016/j.nutres.2020.05.003

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Abstract

The association between intestinal permeability (IP) and body composition remains unclear. The gold standard differential sugar-absorption test is arduous to complete, with zonulin being increasingly used as an independent biomarker of IP. This pilot study aimed to explore the association between small IP, zonulin concentrations and body composition in healthy adults. The urinary lactulose-rhamnose ratio was used to measure small IP. Serum zonulin, lipopolysaccharide (LPS) and high-sensitivity C-reactive protein (hs-CRP) were analyzed in serum. Body composition was measured using dual-energy X-ray absorptiometry and anthropometric measurements were collected. In total, 34 participants were included (12 males, median age 28 years, body mass index 24kg/m2, waist circumference 77cm). No correlation was observed between the lactulose-rhamnose ratio and zonulin (r -0.016, p 0.929). The lactulose-rhamnose ratio displayed a strong positive correlation with LPS (n 22, r 0.536, p 0.018) but did not correlate with body composition measures. Conversely, zonulin displayed a moderate positive correlation with waist circumference (r 0.437, p 0.042) in female participants and hs-CRP (r 0.485, p 0.004) in all participants. These findings raise important considerations for the measurement of small IP, warranting exploration in larger powered studies that address the limitations of the present study.

Details

Title: Serum zonulin measured by enzyme-linked immunosorbent assay may not be a reliable marker of small intestinal permeability in healthy adults
Authors/Creators: O. Tatucu-Babet (Author/Creator) - La Trobe University
A. Forsyth (Author/Creator) - La Trobe University
E. Owen (Author/Creator) - La Trobe University
D. Navarro-Perez (Author/Creator) - La Trobe University
J. Radcliffe (Author/Creator) - La Trobe University
D. Benheim (Author/Creator) - La Trobe University
H. Mendis (Author/Creator) - The University of Melbourne
M. Jois (Author/Creator) - La Trobe University
C. Itsiopoulos (Author/Creator) - La Trobe University
A. Tierney (Author/Creator) - University of Limerick
Publication Details: Nutrition Research, Vol.78, pp.82-92
Publisher: Elsevier Inc.
Identifiers: 991005545041907891
Murdoch Affiliation: Vice Chancellery
Language: English
Resource Type: Journal article

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Collaboration types: Domestic collaboration; International collaboration
Citation topics: 1 Clinical & Life Sciences; 1.120 Inflammatory Bowel Diseases & Infections; 1.120.384 Gut Microbiota
Web Of Science research areas: Nutrition & Dietetics
ESI research areas: Biology & Biochemistry