Journal article
Short‐term hypoxia promotes vascularization in co‐culture system consisting of primary human osteoblasts and outgrowth endothelial cells
Journal of Biomedical Materials Research Part A, Vol.108(1), pp.7-18
2020
Abstract
Prevascularization of tissue constructs before implantation has been developed as a novel and promising concept for successful implantation. Since hypoxia might induce angiogenesis, we have investigated the effects of hypoxic treatment on vascularization by using co‐cultures of primary human osteoblasts (POBs) and outgrowth endothelial cells. Our results show that: (a) repeated short‐term hypoxia (2% O2 for 8 hr), not long‐term hypoxia (2% O2 for 24 hr), over 1 or 2 weeks, significantly enhances microvessel formation in co‐cultures; (b) sustained hypoxia, not short‐term or long‐term hypoxia, causes cytotoxicity in mono‐ and co‐cultures; (c) the expression of some angiogenic and inflammatory factors such as vascular endothelial growth factor, platelet‐derived growth factor subunit B, insulin‐like growth factor 1, interleukin‐8, and early growth response protein 1 increases significantly in hypoxia‐treated POB monoculture and co‐cultures after single or multiple 8‐ or 24‐hr hypoxic treatments; (d) long‐term (24 hr) hypoxic treatment induces more angiogenic inhibitors compared with short‐term hypoxic treatment. Our findings suggest that hypoxia‐induced vascularization/angiogenesis is regulated by a complex balance of angiogenic/antiangiogenic factors, and that repeated short‐term hypoxia, but not repeated long‐term hypoxia, promotes the vascularization and tissue regeneration of bone tissue constructs.
Details
- Title
- Short‐term hypoxia promotes vascularization in co‐culture system consisting of primary human osteoblasts and outgrowth endothelial cells
- Authors/Creators
- B. Ma (Author/Creator)M. Li (Author/Creator)S. Fuchs (Author/Creator)I. Bischoff (Author/Creator)A. Hofmann (Author/Creator)R.E. Unger (Author/Creator)C.J. Kirkpatrick (Author/Creator)
- Publication Details
- Journal of Biomedical Materials Research Part A, Vol.108(1), pp.7-18
- Publisher
- Wiley
- Identifiers
- 991005543250307891
- Copyright
- © 2019 Wiley Periodicals, Inc.
- Murdoch Affiliation
- School of Medical, Molecular and Forensic Sciences
- Language
- English
- Resource Type
- Journal article
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Source: InCites
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- Collaboration types
- Domestic collaboration
- International collaboration
- Citation topics
- 1 Clinical & Life Sciences
- 1.102 Stem Cell Research
- 1.102.170 Angiogenesis
- Web Of Science research areas
- Engineering, Biomedical
- Materials Science, Biomaterials
- ESI research areas
- Materials Science