Journal article
Sites of persistence of lumpy skin disease virus in the genital tract of experimentally infected bulls
Reproduction in Domestic Animals, Vol.45(2), pp.250-255
2010
Abstract
The objectives of this work were to determine the site of persistence of lumpy skin disease virus (LSDV) in bulls shedding the virus in semen for a period longer than 28 days, to determine if the virus is present in all fractions of semen and to study lesions that developed in the genital tract. Six serologically negative postpubertal bulls were experimentally infected with a virulent field isolate of LSDV. The polymerase chain reaction (PCR) was performed on sheath washes, vesicular fluid, supernatant and cell‐rich fractions of semen from day 10 to day 26 postinfection (p.i.). Bulls that were positive by PCR on the whole semen sample collected on day 28 p.i. were slaughtered and tissue samples from their genital tracts submitted for histopathological evaluation, immunoperoxidase staining, virus isolation and PCR. Two of the bulls developed severe lumpy skin disease (LSD) and were found to be shedding viral DNA in their semen on day 28 p.i. Viral DNA was identified in all semen fractions from all bulls, but mostly from the cell‐rich fraction and from the severely affected bulls. The PCR assay was positive on postmortem samples of testes and epididymides from the two severely affected bulls. Virus could be recovered from the testes of these two bulls and from the epididymis of one of them. Immunoperoxidase staining was positive for LSDV staining in sections of testes and epididymides exhibiting necrosis. This study suggests that the testis and epididymis are sites of persistence of LSDV in bulls shedding virus in semen for prolonged periods and revealed that viral DNA is present in all fractions of the ejaculate.
Details
- Title
- Sites of persistence of lumpy skin disease virus in the genital tract of experimentally infected bulls
- Authors/Creators
- C.H. Annandale (Author/Creator) - Onderstepoort Veterinary Academic HospitalP.C. Irons (Author/Creator) - Section Reproduction, Department of Production Animal StudiesV.P. Bagla (Author/Creator) - University of PretoriaU.I. Osuagwuh (Author/Creator) - Section Reproduction, Department of Production Animal StudiesE.H. Venter (Author/Creator) - University of Pretoria
- Publication Details
- Reproduction in Domestic Animals, Vol.45(2), pp.250-255
- Publisher
- Blackwell Publishing Inc.
- Identifiers
- 991005540874207891
- Copyright
- © 2008 The Authors.
- Murdoch Affiliation
- Murdoch University
- Language
- English
- Resource Type
- Journal article
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- Citation topics
- 1 Clinical & Life Sciences
- 1.161 Virology - Identification & Sequencing
- 1.161.1662 Poxvirus Immunology
- Web Of Science research areas
- Agriculture, Dairy & Animal Science
- Reproductive Biology
- Veterinary Sciences
- ESI research areas
- Agricultural Sciences