Journal article
Smoking-induced expression of the GPR15 gene indicates its potential role in chronic inflammatory pathologies
The American Journal of Pathology, Vol.185(11), pp.2898-2906
2015
Abstract
Despite the described clear epigenetic effects of smoking, the effect of smoking on genome-wide gene expression in the blood is obscure. We therefore studied the smoking-induced changes in the gene-expression profile of the peripheral blood. RNA was extracted from the whole blood of 48 individuals with a detailed smoking history (24 never-smokers, 16 smokers, and 8 ex-smokers). Gene-expression profiles were evaluated with RNA sequencing, and results were analyzed separately in 24 men and 24 women. In the male smokers, 13 genes were statistically significantly (false-discovery rate <0.1) differentially expressed; in female smokers, 5 genes. Although most of the differentially expressed genes were different between the male and female smokers, the G-protein–coupled receptor 15 gene (GPR15) was differentially expressed in both male and female smokers compared with never-smokers. Analysis of GPR15 methylation identified significantly greater hypomethylation in smokers compared with that in never-smokers. GPR15 is the chemoattractant receptor that regulates T-cell migration and immunity. Up-regulation of GPR15 could explain to some extent the health hazards of smoking with regard to chronic inflammatory diseases.
Details
- Title
- Smoking-induced expression of the GPR15 gene indicates its potential role in chronic inflammatory pathologies
- Authors/Creators
- G. Kõks (Author/Creator) - University of TartuM-L Uudelepp (Author/Creator) - Tartu University HospitalM. Limbach (Author/Creator) - University of TartuP. Peterson (Author/Creator) - University of TartuE. Reimann (Author/Creator) - University of TartuS. Kõks (Author/Creator) - Estonian University of Life Sciences
- Publication Details
- The American Journal of Pathology, Vol.185(11), pp.2898-2906
- Publisher
- Elsevier
- Identifiers
- 991005540307907891
- Copyright
- © 2015 American Society for Investigative Pathology.
- Murdoch Affiliation
- Murdoch University
- Language
- English
- Resource Type
- Journal article
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Source: InCites
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- Collaboration types
- Domestic collaboration
- Citation topics
- 1 Clinical & Life Sciences
- 1.54 Molecular & Cell Biology - Genetics
- 1.54.100 Epigenetic Regulation
- Web Of Science research areas
- Pathology
- ESI research areas
- Clinical Medicine