Journal article
Somatic hybridization of amino acid analogue-resistant cell lines of potato (Solanum tuberosum L.) by electrofusion
Theoretical and Applied Genetics, Vol.73(3), pp.451-458
1987
Abstract
Intraspecific somatic hybridization between amino acid analogue-resistant cell lines of potato (Solanum tuberosum L.) has been carried out following electrofusion of protoplasts. In initial analytical electrofusion experiments (1 mm electrode separation) optimal fusion conditions were determined by changing the fusion medium (addition of Ca and/or spermine) and the electrical parameters. Subsequently, in large scale experiments, cell suspension protoplasts of aec-1, a variant resistant to AEC, were fused with the same type of protoplasts of 5mt-26 or 5mt-27, both variants resistant to 5MT and cross-resistant to 3 FT. After an extensive selection procedure only somatic hybrid lines of aec-1 + 5mt-26 were obtained. The resistance traits of aec-1 and 5mt-26 were expressed fully, indicating that the variant characters involved are transmitted dominantly. Quantitative examination of the free amino acid content revealed characteristics of both the parental cell lines in most of the somatic hybrids. However, initially selected double resistant colonies from fusions of aec-1 + 5mt-27 lines appeared not to be somatic hybrids.
Details
- Title
- Somatic hybridization of amino acid analogue-resistant cell lines of potato (Solanum tuberosum L.) by electrofusion
- Authors/Creators
- S.E. De Vries (Author/Creator) - University of GroningenE. Jacobsen (Author/Creator) - University of GroningenM.G.K. Jones (Author/Creator) - Rothamsted ResearchA.E.H.M. Loonen (Author/Creator) - University of GroningenM.J. Tempelaar (Author/Creator) - University of GroningenJ. Wijbrandi (Author/Creator) - University of GroningenW.J. Feenstra (Author/Creator) - University of Groningen
- Publication Details
- Theoretical and Applied Genetics, Vol.73(3), pp.451-458
- Publisher
- Springer Verlag
- Identifiers
- 991005540212807891
- Murdoch Affiliation
- Murdoch University
- Language
- English
- Resource Type
- Journal article
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- Collaboration types
- Domestic collaboration
- International collaboration
- Citation topics
- 3 Agriculture, Environment & Ecology
- 3.4 Crop Science
- 3.4.119 Micropropagation
- Web Of Science research areas
- Agronomy
- Genetics & Heredity
- Horticulture
- Plant Sciences
- ESI research areas
- Agricultural Sciences