Spatial and temporal coordination of expression of immune response genes during Pseudomonas infection of horseshoe crab, Carcinoscorpius rotundicauda

J.L. Ding; K.C. Tan; S. Thangamani; N. Kusuma; W.K. Seow; T.H.H. Bui; J. Wang; B. Ho

doi:10.1038/sj.gene.6364240

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Spatial and temporal coordination of expression of immune response genes during Pseudomonas infection of horseshoe crab, Carcinoscorpius rotundicauda

Journal article

Peer reviewed

Spatial and temporal coordination of expression of immune response genes during Pseudomonas infection of horseshoe crab, Carcinoscorpius rotundicauda

J.L. Ding, K.C. Tan, S. Thangamani, N. Kusuma, W.K. Seow, T.H.H. Bui, J. Wang and B. Ho

Genes and Immunity, Vol.6(7), pp.557-574

2005

DOI: https://doi.org/10.1038/sj.gene.6364240

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Abstract

Knowledge on how genes are turned on/off during infection and immunity is lacking. Here, we report the coregulation of diverse clusters of functionally related immune response genes in a horseshoe crab, Carcinoscorpius rotundicauda. Expressed sequence tag (EST) clusters for frontline immune defense, cell signalling, apoptosis and stress response genes were expressed or repressed spatio-temporally during the acute phase of Pseudomonas infection. An infection time course monitored by virtual Northern evaluation indicates upregulation of genes in blood cells (amebocytes) at 3-h postinfection, whereas most of the hepatopancreas genes remained downregulated over 72h of infection. Thus, the two tissues orchestrate a coordinated and timely response to infection. The hepatopancreas probably immunomodulates the expression of other genes and serves as a reservoir for later response, if/when chronic infection ensues. On the other hand, being the first to encounter pathogens, we reasoned that amebocytes would respond acutely to infection. Besides acute transactivation of the immune genes, the amebocytes maintained morphological integrity, indicating their ability to synthesise and store/secrete the immune proteins and effectors to sustain the frontline innate immune defense, while simultaneously elicit complement-mediated phagocytosis of the invading pathogen. Our results show that the immune response against Pseudomonas infection is spatially and temporally coordinated.

Details

Title: Spatial and temporal coordination of expression of immune response genes during Pseudomonas infection of horseshoe crab, Carcinoscorpius rotundicauda
Authors/Creators: J.L. Ding (Author/Creator) - National University of Singapore
K.C. Tan (Author/Creator) - Murdoch University
S. Thangamani (Author/Creator) - National University of Singapore
N. Kusuma (Author/Creator) - National University of Singapore
W.K. Seow (Author/Creator) - National University of Singapore
T.H.H. Bui (Author/Creator) - National University of Singapore
J. Wang (Author/Creator) - National University of Singapore
B. Ho (Author/Creator) - National University of Singapore
Publication Details: Genes and Immunity, Vol.6(7), pp.557-574
Publisher: Nature Publishing Group
Identifiers: 991005541687207891
Copyright: © 2005 Nature Publishing Group.
Murdoch Affiliation: Australian Centre for Necrotrophic Fungal Pathogens
Language: English
Resource Type: Journal article

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Citation topics: 3 Agriculture, Environment & Ecology; 3.32 Entomology; 3.32.1508 Invertebrate Immunity
Web Of Science research areas: Genetics & Heredity; Immunology
ESI research areas: Molecular Biology & Genetics