Abstract
Spatial omics technologies enable unraveling of single-cell heterogeneity and characterizing diverse cell types in plants while preserving their spatial arrangement.Spatial transcriptomics facilitates visualization and quantification of gene expression across the entire transcriptome in plant tissue cryosections, using strategies such as barcoded oligo(dT) arrays and high-throughput sequencing.Spatial proteomics and metabolomics are advancing in resolution, field of view, and cost-efficiency. Achieving single-cell resolution in plants requires overcoming challenges in both experimental techniques and computational analysis.Spatially resolved multiomics profiling and 3D spatial omics hold potential to shape future crop improvement strategies by providing a holistic understanding of molecular and cellular features that control agronomically important traits.
Plant cells communicate information to regulate developmental processes and respond to environmental stresses. This communication spans various ‘omics’ layers within a cell and operates through intricate regulatory networks. The emergence of spatial omics presents a promising approach to thoroughly analyze cells, allowing the combined analysis of diverse modalities either in parallel or on the same tissue section. Here, we provide an overview of recent advancements in spatial omics and delineate scientific discoveries in plant research enabled by these technologies. We delve into experimental and computational challenges and outline strategies to navigate these challenges for advancing breeding efforts. With ongoing insightful discoveries and improved accessibility, spatial omics stands on the brink of playing a crucial role in designing future crops.
Plant cells communicate information to regulate developmental processes and respond to environmental stresses. This communication spans various ‘omics’ layers within a cell and operates through intricate regulatory networks. The emergence of spatial omics presents a promising approach to thoroughly analyze cells, allowing the combined analysis of diverse modalities either in parallel or on the same tissue section. Here, we provide an overview of recent advancements in spatial omics and delineate scientific discoveries in plant research enabled by these technologies. We delve into experimental and computational challenges and outline strategies to navigate these challenges for advancing breeding efforts. With ongoing insightful discoveries and improved accessibility, spatial omics stands on the brink of playing a crucial role in designing future crops.