Specific alternative HOX11 transcripts are expressed in paediatric neural tumours and T-cell acute lymphoblastic leukaemia

P.M. Watt; K. Hoffmann; W.K. Greene; R.L. Brake; J. Ford; U.R. Kees

doi:10.1016/j.gene.2003.09.001

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Specific alternative HOX11 transcripts are expressed in paediatric neural tumours and T-cell acute lymphoblastic leukaemia

Journal article

Peer reviewed

Specific alternative HOX11 transcripts are expressed in paediatric neural tumours and T-cell acute lymphoblastic leukaemia

P.M. Watt, K. Hoffmann, W.K. Greene, R.L. Brake, J. Ford and U.R. Kees

Gene, Vol.323(1-2), pp.89-99

2003

DOI: https://doi.org/10.1016/j.gene.2003.09.001

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Abstract

HOX11 is a proto-oncogene, which is silent in normal mature T-cells, while being aberrantly activated in T-cell acute lymphoblastic leukaemia (T-ALL) by translocations t(10;14)(q24;q11) or t(7;10)(q35;q24). Although many oncogenes are expressed in alternative forms in cancer, thus far, only one form of the human HOX11 transcript has been reported. We describe here the identification of three alternative transcripts of the HOX11 proto-oncogene, expressed in primary T-ALL specimens. Using rapid amplification of cDNA ends (RACE) and targeted RT-PCR, we have sequenced 23 individual cDNA clones characterising these novel transcripts. Northern hybridisation identified particular novel exons expressed in T-ALL, which are not expressed in normal T-cells. To date, aberrant expression of HOX11 has only been associated with leukaemia. Our survey of a range of neuroblastoma and primitive neuroectodermal tumour (PNET) cell lines demonstrated the expression of these novel HOX11 transcripts in tumours of neural origin, while their expression was not detected in normal brain tissues. Strikingly, the dominant transcript in these neural tumour cell lines is more than 1 kb larger than the dominant transcript in T-ALL. These observations, combined with sequence data from several EST clones derived from medulloblastoma cDNA libraries, support a new hypothesis that HOX11 may also function as a neural oncogene or brain tumour marker.

Details

Title: Specific alternative HOX11 transcripts are expressed in paediatric neural tumours and T-cell acute lymphoblastic leukaemia
Authors/Creators: P.M. Watt (Author/Creator) - The Kids Research Institute Australia
K. Hoffmann (Author/Creator) - The Kids Research Institute Australia
W.K. Greene (Author/Creator) - Murdoch University
R.L. Brake (Author/Creator) - The Kids Research Institute Australia
J. Ford (Author/Creator) - The Kids Research Institute Australia
U.R. Kees (Author/Creator) - The Kids Research Institute Australia
Publication Details: Gene, Vol.323(1-2), pp.89-99
Publisher: Elsevier BV
Identifiers: 991005541181407891
Copyright: © 2003 Elsevier B.V.
Murdoch Affiliation: School of Veterinary and Biomedical Sciences
Language: English
Resource Type: Journal article

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Collaboration types: Domestic collaboration
Citation topics: 1 Clinical & Life Sciences; 1.108 Molecular & Cell Biology - Cancer & Development; 1.108.207 Neural Crest
Web Of Science research areas: Genetics & Heredity
ESI research areas: Molecular Biology & Genetics