Journal article
Specific and genotypic identification of Cryptosporidium from a broad range of host species by nonisotopic SSCP analysis of nuclear ribosomal DNA
Electrophoresis, Vol.28(16), pp.2818-2825
2007
Abstract
The accurate identification of Cryptosporidium (Protozoa: Apicomplexa) species and genotypes is central to the understanding of the transmission and to the diagnosis and control of cryptosporidiosis. In this study, we demonstrate the effectiveness of nonisotopic SSCP analysis of a ∼300 bp region of the small subunit (pSSU) of ribosomal DNA for the specific identification of and delineation among 18 different Cryptosporidium species and genotypes from a wide range of hosts. This mutation scanning approach allowed the rapid and reliable differentiation between species/genotypes differing by as little as 1.3% in the pSSU sequence, with the capacity to detect point mutations. The present findings confirm the usefulness of this tool for the rapid genetic screening of Cryptosporidium samples from any host species, providing a foundation for detailed systematic, epidemiological and ecological studies. Although applied herein to pSSU, this low cost approach should be applicable to a wide range of genetic loci for population genetic investigations of Cryptosporidium.
Details
- Title
- Specific and genotypic identification of Cryptosporidium from a broad range of host species by nonisotopic SSCP analysis of nuclear ribosomal DNA
- Authors/Creators
- A.R. Jex (Author/Creator) - The University of MelbourneU.M. Ryan (Author/Creator) - Murdoch UniversityJ. Ng (Author/Creator) - Murdoch UniversityB.E. Campbell (Author/Creator) - The University of MelbourneL. Xiao (Author/Creator) - Centers for Disease Control and PreventionM. Stevens (Author/Creator) - Melbourne WaterR.B. Gasser (Author/Creator) - The University of Melbourne
- Publication Details
- Electrophoresis, Vol.28(16), pp.2818-2825
- Publisher
- John Wiley & Sons Ltd
- Identifiers
- 991005542854207891
- Copyright
- © 2007 Wiley-VCH Verlag GmbH & Co. KGaA, Weinheim
- Murdoch Affiliation
- School of Veterinary and Biomedical Sciences
- Language
- English
- Resource Type
- Journal article
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- Collaboration types
- Domestic collaboration
- International collaboration
- Citation topics
- 1 Clinical & Life Sciences
- 1.246 Diarrheal Diseases
- 1.246.985 Cryptosporidium
- Web Of Science research areas
- Biochemical Research Methods
- Chemistry, Analytical
- ESI research areas
- Chemistry