Journal article
Stable transformation of Rhizoctonia solani with a modified hygromycin resistance gene
Australasian Plant Pathology, Vol.38(1), pp.79-84
2009
Abstract
A vector for Agrobacterium tumefaciens-mediated transformation of basidiomycetes was constructed by insertion of a modified hygromycin resistance gene into the plant binary vector pBin19. The hygromycin coding region is flanked by a basidiomycete promoter and terminator. Isolates from different anastomosis groupings (AG) of the phytopathogenic fungus Rhizoctonia solani (Kuhn) were transformed with this vector using A. tumefaciens. Hygromycin-resistant transformants were isolated from a single AG6 isolate, but not from AG3 or AG4 isolates. Of six transformants isolated, five showed enhanced growth on agar containing either 25 or 50 μg/mL hygromycin. However, as the hygromycin concentration increased, the difference between the transformants and the control reduced, until at 100 μg/mL there was no difference. The resistance phenotype was retained through repeated subcultures on non-selective media. The presence of the gene in the transformants was confirmed by PCR analysis and Southern hybridisation.
Details
- Title
- Stable transformation of Rhizoctonia solani with a modified hygromycin resistance gene
- Authors/Creators
- J. Wu (Author/Creator)P.A. O'Brien (Author/Creator)
- Publication Details
- Australasian Plant Pathology, Vol.38(1), pp.79-84
- Publisher
- CSIRO Publishing
- Identifiers
- 991005545169907891
- Copyright
- 2009 Australasian Plant Pathology Society
- Murdoch Affiliation
- School of Biological Sciences and Biotechnology
- Language
- English
- Resource Type
- Journal article
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- Citation topics
- 1 Clinical & Life Sciences
- 1.148 Medical Mycology
- 1.148.240 Saccharomyces Cerevisiae
- Web Of Science research areas
- Plant Sciences
- ESI research areas
- Plant & Animal Science