Journal article
Stable transformation of sugarbeet protoplasts by electroporation
Plant Cell Reports, Vol.8(2), pp.71-74
1989
Abstract
Conditions were optimized for the culture, antibiotic selection and stable transformation by electroporation of suspension culture protoplasts of sugarbeet,Beta vulgaris L.. Highest plating efficiencies (up to 65% at day 21) were obtained if protoplasts were cultured in PGO salts (de Greef and Jacobs, 1979) supplemented with 0.1 mg/1 2,4-D, 0.01 mg/l BAP and 9% mannitol, and in 0.6% agarose rather than in liquid medium. Sensitivity to kanamycin also depended on whether protoplasts were cultured in liquid or agarose medium. Stable transformation of protoplast-derived colonies, as determined by resistance to kanamycin and Southern blot analysis, was achieved by electroporation using both rectangular and exponentially-decaying pulses.
Details
- Title
- Stable transformation of sugarbeet protoplasts by electroporation
- Authors/Creators
- K. Lindsey (Author/Creator)M.G.K. Jones (Author/Creator)
- Publication Details
- Plant Cell Reports, Vol.8(2), pp.71-74
- Publisher
- Springer Verlag
- Identifiers
- 991005542441807891
- Copyright
- © 1989 Springer-Verlag
- Murdoch Affiliation
- Murdoch University
- Language
- English
- Resource Type
- Journal article
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- Citation topics
- 3 Agriculture, Environment & Ecology
- 3.4 Crop Science
- 3.4.119 Micropropagation
- Web Of Science research areas
- Plant Sciences
- ESI research areas
- Plant & Animal Science