Stepping towards highly flexible aptamers: Enzymatic recognition studies of unlocked nucleic acid nucleotides

C. Dubois; M.A. Campbell; S.L. Edwards; J. Wengel; R.N. Veedu

doi:10.1039/C2CC31316B

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Stepping towards highly flexible aptamers: Enzymatic recognition studies of unlocked nucleic acid nucleotides

Journal article

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Stepping towards highly flexible aptamers: Enzymatic recognition studies of unlocked nucleic acid nucleotides

C. Dubois, M.A. Campbell, S.L. Edwards, J. Wengel and R.N. Veedu

Chemical Communications, Vol.48(44)

2012

DOI: https://doi.org/10.1039/C2CC31316B

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Abstract

Enzymatic recognition of unlocked nucleic acid (UNA) nucleotides was successfully accomplished. Therminator DNA polymerase was found to be an efficient enzyme in primer extension reactions. Polymerase chain reaction (PCR) amplification of a 81 mer UNA-modified DNA library was efficiently achieved by KOD DNA polymerase.

Details

Title: Stepping towards highly flexible aptamers: Enzymatic recognition studies of unlocked nucleic acid nucleotides
Authors/Creators: C. Dubois (Author/Creator)
M.A. Campbell (Author/Creator)
S.L. Edwards (Author/Creator)
J. Wengel (Author/Creator)
R.N. Veedu (Author/Creator)
Publication Details: Chemical Communications, Vol.48(44)
Publisher: Royal Society of Chemistry
Identifiers: 991005540183407891
Murdoch Affiliation: Murdoch University
Language: English
Resource Type: Journal article

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