Journal article
Stimulation of human EBV-and CMV-specific cytolytic effector function using allogeneic HLA molecules
The Journal of Immunology, Vol.189(10), pp.4825-4831
2012
Abstract
Viral infection is a major cause of morbidity and mortality, and there are few therapeutic options available to augment a virusspecific T cell response. Although allo-HLA cross-reactivity from virus-specific memory T cells is common, it is unclear whether priming with specific allogeneic cells could conversely elicit a viral peptide/self-HLA restricted cytotoxic T cell response in humans. First, we used the previously described allo-HLA-B*44:0 - cross-reactivity of EBV peptide/HLA-B8 restricted T cells, to determine whether allogeneic HLA stimulation can elicit a cytolytic immune response against EBV. HLA-B8 + HLA-B44 - EBV-seropositive PBMCs were stimulated with either HLA-B*44:02 + or HLA-B*44:03 + mismatched irradiated PBMCs in a 7-10 d MLR. The allo-HLA stimulated responder cells were then evaluated for cytotoxicity using EBV peptide loaded autologous target cells and unloaded HLA-B8 + EBV LCL target cells. PBMCs from EBV-seropositive donors gained EBV-specific cytolytic effector function following specific allo-HLA stimulation. Finally, we also elicited cytolytic CMV-specific responses using specific allogeneic cell stimulation, to confirm that this technique can be used to elicit viral peptide/self-HLA restricted responses even from nonpublic TCR responses. Allogeneic cell stimulation used as a cell therapy may be a potential tool to augment an antiviral T cell response in patients with EBV or CMV infection.
Details
- Title
- Stimulation of human EBV-and CMV-specific cytolytic effector function using allogeneic HLA molecules
- Authors/Creators
- L.J. D'Orsogna (Author/Creator)H. van den Heuvel (Author/Creator)E.M.W. van der Meer-Prins (Author/Creator)D.L. Roelen (Author/Creator)I.I.N. Doxiadis (Author/Creator)F.H.J. Claas (Author/Creator)
- Publication Details
- The Journal of Immunology, Vol.189(10), pp.4825-4831
- Publisher
- American Association of Immunologists
- Identifiers
- 991005544892507891
- Copyright
- © 2012 by The American Association of Immunologists, Inc
- Murdoch Affiliation
- Institute for Immunology and Infectious Diseases
- Language
- English
- Resource Type
- Journal article
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- Collaboration types
- Domestic collaboration
- International collaboration
- Citation topics
- 1 Clinical & Life Sciences
- 1.6 Immunology
- 1.6.127 T Cell Regulation
- Web Of Science research areas
- Immunology
- ESI research areas
- Immunology