Journal article
Streptomyces misionensis PESB-25 Produces a thermoacidophilic endoglucanase using sugarcane bagasse and corn steep liquor as the sole organic substrates
BioMed research international, Vol.2013, 584207
2013
PMID: 23586048
Abstract
Streptomyces misionensis strain PESB-25 was screened and selected for its ability to secrete cellulases. Cells were grown in a liquid medium containing sugarcane bagasse (SCB) as carbon source and corn steep liquor (CSL) as nitrogen source, whose concentrations were optimized using response surface methodology (RSM). A peak of endoglucanase accumulation (1.01 U·mL−1) was observed in a medium with SCB 1.0% (w/v) and CSL 1.2% (w/v) within three days of cultivation. S. misionensis PESB-25 endoglucanase activity was thermoacidophilic with optimum pH and temperature range of 3.0 to 3.6 and 62° to 70°C, respectively. In these conditions, values of 1.54 U mL−1 of endoglucanase activity were observed. Moreover, Mn2+ was demonstrated to have a hyperactivating effect on the enzyme. In the presence of MnSO4 (8 mM), the enzyme activity increased threefold, up to 4.34 U·mL−1. Mn2+ also improved endoglucanase stability as the catalyst retained almost full activity upon incubation at 50°C for 4 h, while in the absence of Mn2+, enzyme activity decreased by 50% in this same period. Three protein bands with endoglucanase activity and apparent molecular masses of 12, 48.5 and 119.5 kDa were detected by zymogram.
Details
- Title
- Streptomyces misionensis PESB-25 Produces a thermoacidophilic endoglucanase using sugarcane bagasse and corn steep liquor as the sole organic substrates
- Authors/Creators
- Marcella Novaes Franco-Cirigliano - Departamento de Microbiologia Geral, Instituto de Microbiologia Paulo de Góes, Universidade Federal Do Rio de Janeiro (UFRJ), BrazilRaquel De Carvalho Rezende - Departamento de Microbiologia Geral, Instituto de Microbiologia Paulo de Góes, Universidade Federal Do Rio de Janeiro (UFRJ), BrazilMônica Pires Gravina-Oliveira - Departamento de Microbiologia Geral, Instituto de Microbiologia Paulo de Góes, Universidade Federal Do Rio de Janeiro (UFRJ), BrazilPedro Henrique Freitas Pereira - Departamento de Microbiologia Geral, Instituto de Microbiologia Paulo de Góes, Universidade Federal Do Rio de Janeiro (UFRJ), BrazilRodrigo Pires Do Nascimento - Departamento de Engenharia Bioquímica, Escola de Química, Universidade Federal Do Rio de Janeiro (UFRJ), BrazilElba Pinto Da Silva Bon - Departamento de Bioquímica, Instituto de Química, Universidade Federal Do Rio de Janeiro (UFRJ), BrazilAndrew Macrae - Departamento de Microbiologia Geral, Instituto de Microbiologia Paulo de Góes, Universidade Federal Do Rio de Janeiro (UFRJ), BrazilRosalie Reed Rodrigues Coelho - Departamento de Microbiologia Geral, Instituto de Microbiologia Paulo de Góes, Universidade Federal Do Rio de Janeiro (UFRJ), Brazil
- Publication Details
- BioMed research international, Vol.2013, 584207
- Identifiers
- 991005894543607891
- Copyright
- © 2013 Marcella Novaes Franco-Cirigliano et al
- Murdoch Affiliation
- Food Futures Institute
- Language
- English
- Resource Type
- Journal article
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