Strongyloides ratti: Mitochondrial enzyme activities of the classical electron transport pathway in the infective (L3) larvae

A. Armson; W.B. Grubb; A.H.W. Mendis

doi:10.1016/0020-7519(94)E0062-R

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Strongyloides ratti: Mitochondrial enzyme activities of the classical electron transport pathway in the infective (L3) larvae

Journal article

Peer reviewed

Strongyloides ratti: Mitochondrial enzyme activities of the classical electron transport pathway in the infective (L3) larvae

A. Armson, W.B. Grubb and A.H.W. Mendis

International Journal for Parasitology, Vol.25(2), pp.257-260

1995

DOI: https://doi.org/10.1016/0020-7519(94)E0062-R

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Abstract

Submitochondrial particles prepared from S. ratti L3 larvae exhibited NADH-oxidase (NOX), NADH-ferricyanide reductase (NFR), NADH-cytochrome-c-reductase (NCR), succinate-cytochrome-c-reductase (SCR), and cytochrome-aa3-oxidase activities of 2.1 ± 0.3, 8.9 ± 1.3, 0.6 ± 0.1, 1.0 ± 0.2 and 1.2 ± 0.3 nm min−1 mg protein−1 respectively, at 37°C. The NCR and NOX activities were 39.3% and 23.5% of the NFR activity, suggesting the occurrence of a rate-limiting step or bifurcation of the respiratory electron transport (RET) pathway on the oxygen-side of RET-Complex I. The NCR activity was 50% that of cytochrome-aa3-oxidase activity which suggests partitioning of electron flow at the level of RET-Complex III and/or the quinone-function. Antimycin A and rotesone but not 2-thenoyl trifluoreacetone (TTFA) inhibited NCR activity, the EC50 values were 3.6 × 10−6m, 3.7 × 10−7m, respectively. SCR activity was inhibited by antimycin A (EC50 = 3.8 × 10−6m) and TTFA (EC50 = 2.8 × 10−5m) but not by rotenone. The results suggest that presence of classical and alternate RET-pathways in S. ratti L3 larvae.

Details

Title: Strongyloides ratti: Mitochondrial enzyme activities of the classical electron transport pathway in the infective (L3) larvae
Authors/Creators: A. Armson (Author/Creator) - Curtin University
W.B. Grubb (Author/Creator) - Curtin University
A.H.W. Mendis (Author/Creator) - Curtin University
Publication Details: International Journal for Parasitology, Vol.25(2), pp.257-260
Publisher: Elsevier BV
Identifiers: 991005541455707891
Copyright: 1995 Australian Society for Parasitology
Murdoch Affiliation: Murdoch University
Language: English
Resource Type: Journal article

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Citation topics: 1 Clinical & Life Sciences; 1.163 Parasitology - General; 1.163.1022 Anthelmintic Resistance
Web Of Science research areas: Parasitology
ESI research areas: Microbiology