Journal article
Study on effect of extraction techniques and seed coat on proteomic distribution and cheese production from soybean milk
Molecules, Vol.25(14), Article 3237
2020
Abstract
Soybean-based food products are a major source of protein. In the present study, proteins in soybean milk from seeds of the cultivar Bunya (Glycine max) were extracted using the cheesecloth and the centrifuge methods. The milk was produced through mechanical crushing of both whole and split seeds in water. Following separation by either the cheesecloth or centrifuge, proteins were isolated from the soybean milk by using thiourea/urea solubilisation and then separated them using two-dimensional polyacrylamide gel electrophoresis. The isolated proteins were identified by mass spectrometry. A total of 97 spots were identified including 49 that displayed different abundances. Of the two separation techniques, centrifuge separation gave higher protein extraction and more intense protein spots than cheesecloth separation. Eleven of the β-subunits of β-conglycinin, three of the α-subunits of β-conglycinin, and four of the mutant glycinin showed different levels of abundances between separation techniques, which might be related to subsequent cheese quality. Notably, split-seed soybean milk has less allergenic proteins with four α-subunits of β-conglycinin compared to whole-seed milk with eight of those proteins. The sensory evaluation showed that the cheese produced from split-soybean milk received higher consumer preferences compared to that of whole seed, which could be explained by their proteomic differences. The demonstrated reference map for whole and split-seed soybean milk could be further utilized in the research related to soybean cheesemaking.
Details
- Title
- Study on effect of extraction techniques and seed coat on proteomic distribution and cheese production from soybean milk
- Authors/Creators
- N. Al-Saedi (Author/Creator) - Murdoch UniversityM. Agarwal (Author/Creator) - Murdoch UniversityW. Ma (Author/Creator) - Murdoch UniversityS. Islam (Author/Creator) - Murdoch UniversityY. Ren (Author/Creator) - Murdoch University
- Publication Details
- Molecules, Vol.25(14), Article 3237
- Publisher
- MDPI
- Identifiers
- 991005541368007891
- Copyright
- © 2020 by the authors
- Murdoch Affiliation
- College of Science, Health, Engineering and Education
- Language
- English
- Resource Type
- Journal article
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- Collaboration types
- Domestic collaboration
- International collaboration
- Citation topics
- 3 Agriculture, Environment & Ecology
- 3.85 Food Science & Technology
- 3.85.553 Protein-Stabilized Emulsions
- Web Of Science research areas
- Biochemistry & Molecular Biology
- Chemistry, Multidisciplinary
- ESI research areas
- Chemistry