Journal article
Subtractive hybridization of cDNA from small amounts of plant tissue
Molecular Biotechnology, Vol.8(1), pp.7-12
1997
Abstract
A subtractive hybridization method is described that allows the generation of a subtractive gene library from small amounts of plant or other eukaryotic tissues. The method uses paramagnetic oligo-dT beads to capture poly-adenylated mRNA and to synthesize the complementary cDNA on a solid support. The use of magnetic beads facilitates the change of reaction buffers and the removal of primers and minimizes yield losses. Subtracted material obtained from this method can either be cloned directly or used to screen a specific library.
Details
- Title
- Subtractive hybridization of cDNA from small amounts of plant tissue
- Authors/Creators
- T. Heinrich (Author/Creator) - Murdoch UniversityS. Washer (Author/Creator) - Murdoch UniversityJ. Marshall (Author/Creator) - New Zealand Institute for Crop and Food ResearchM.G.K. Jones (Author/Creator) - Murdoch UniversityR.H. Potter (Author/Creator) - Murdoch University
- Publication Details
- Molecular Biotechnology, Vol.8(1), pp.7-12
- Publisher
- Springer
- Identifiers
- 991005542031807891
- Copyright
- © Humana Press Inc. 1997
- Murdoch Affiliation
- State Agricultural Biotechnology Centre
- Language
- English
- Resource Type
- Journal article
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- Citation topics
- 1 Clinical & Life Sciences
- 1.54 Molecular & Cell Biology - Genetics
- 1.54.1454 PCR Techniques
- Web Of Science research areas
- Biochemistry & Molecular Biology
- Biotechnology & Applied Microbiology
- ESI research areas
- Biology & Biochemistry