Sulforaphane enhanced proliferation of porcine satellite cells via epigenetic augmentation of SMAD7

R. Zhang; C. Neuhoff; Q. Yang; M.U. Çınar; M.J. Uddin; E. Tholen; K. Schellander; D. Tesfaye

doi:10.3390/ani12111365

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Sulforaphane enhanced proliferation of porcine satellite cells via epigenetic augmentation of SMAD7

Journal article

Open access

Peer reviewed

Sulforaphane enhanced proliferation of porcine satellite cells via epigenetic augmentation of SMAD7

R. Zhang, C. Neuhoff, Q. Yang, M.U. Çınar, M.J. Uddin, E. Tholen, K. Schellander and D. Tesfaye

Animals, Vol.12(11), Article 1365

2022

DOI: https://doi.org/10.3390/ani12111365

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Abstract

Satellite cells take an indispensable place in skeletal muscle regeneration, maintenance, and growth. However, only limited works have investigated effects of dietary compounds on the proliferation of porcine satellite cells (PSCs) and related mechanisms. Sulforaphane (SFN) at multiple levels was applied to PSCs. The PSCs’ viability and HDAC activity were measured with a WST-1 cell proliferation kit and Color-de-Lys® HDAC colorimetric activity assay kit. Gene expression and epigenetics modification were tested with qRT-PCR, Western blot, bisulfite sequencing, and ChIP-qPCR. This study found that SFN enhanced PSC proliferation and altered mRNA expression levels of myogenic regulatory factors. In addition, SFN inhibited histone deacetylase (HDAC) activity, disturbed mRNA levels of HDAC family members, and elevated acetylated histone H3 and H4 abundance in PSCs. Furthermore, both mRNA and protein levels of the Smad family member 7 (SMAD7) in PSCs were upregulated after SFN treatment. Finally, it was found that SFN increased the acetylation level of histone H4 in the SMAD7 promoter, decreased the expression of microRNAs, including ssc-miR-15a, ssc-miR-15b, ssc-miR-92a, ssc-miR-17-5p, ssc-miR-20a-5p, and ssc-miR-106a, targeting SMAD7, but did not impact on the SMAD7 promoter’s methylation status in PSCs. In summary, SFN was found to boost PSC proliferation and epigenetically increase porcine SMAD7 expression, which indicates a potential application of SFN in modulation of skeletal muscle growth.

Details

Title: Sulforaphane enhanced proliferation of porcine satellite cells via epigenetic augmentation of SMAD7
Authors/Creators: R. Zhang (Author/Creator) - Chengdu University
C. Neuhoff (Author/Creator) - University of Bonn
Q. Yang (Author/Creator) - University of Bonn
M.U. Çınar (Author/Creator)
M.J. Uddin (Author/Creator) - University of Bonn
E. Tholen (Author/Creator) - University of Bonn
K. Schellander (Author/Creator) - University of Bonn
D. Tesfaye (Author/Creator) - University of Bonn
Publication Details: Animals, Vol.12(11), Article 1365
Publisher: MDPI
Identifiers: 991005542651607891
Copyright: © 2022 by the authors
Murdoch Affiliation: School of Veterinary Medicine
Language: English
Resource Type: Journal article

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Collaboration types: Domestic collaboration; International collaboration
Citation topics: 1 Clinical & Life Sciences; 1.127 Molecular & Cell Biology - Pharmacology; 1.127.1323 NRF2
Web Of Science research areas: Agriculture, Dairy & Animal Science; Veterinary Sciences; Zoology
ESI research areas: Plant & Animal Science