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CC BY V4.0, Open Access
Journal article
Synchrotron-Based Techniques Shed Light on Mechanisms of Plant Sensitivity and Tolerance to High Manganese in the Root Environment
Plant physiology (Bethesda), Vol.169(3), pp.2006-2020
2015
PMID: 26395840
Abstract
Plant species differ in response to high available manganese (Mn), but the mechanisms of sensitivity and tolerance are poorly understood. In solution culture, greater than or equal to 30 mu M Mn decreased the growth of soybean (Glycine max), but white lupin (Lupinus albus), narrow-leafed lupin (Lupin angustifolius), and sunflower (Helianthus annuus) grew well at 100 mM Mn. Differences in species' tolerance to high Mn could not be explained simply by differences in root, stem, or leaf Mn status, being 8.6, 17.1, 6.8, and 9.5 mmol kg(-1) leaf fresh mass at 100 mM Mn. Furthermore, x-ray absorption near edge structure analyses identified the predominance of Mn(II), bound mostly to malate or citrate, in roots and stems of all four species. Rather, differences in tolerance were due to variations in Mn distribution and speciation within leaves. In Mn-sensitive soybean, in situ analysis of fresh leaves using x-ray fluorescence microscopy combined with x-ray absorption near edge structure showed high Mn in the veins, and manganite [Mn(III)] accumulated in necrotic lesions apparently through low Mn sequestration in vacuoles or other vesicles. In the two lupin species, most Mn accumulated in vacuoles as either soluble Mn(II) malate or citrate. In sunflower, Mn was sequestered as manganite at the base of nonglandular trichomes. Hence, tolerance to high Mn was ascribed to effective sinks for Mn in leaves, as Mn(II) within vacuoles or through oxidation of Mn(II) to Mn(III) in trichomes. These two mechanisms prevented Mn accumulation in the cytoplasm and apoplast, thereby ensuring tolerance to high Mn in the root environment.
Details
- Title
- Synchrotron-Based Techniques Shed Light on Mechanisms of Plant Sensitivity and Tolerance to High Manganese in the Root Environment
- Authors/Creators
- F. Pax C. Blamey - The University of QueenslandMaria C. Hernandez-Soriano - Univ Queensland, Sch Agr & Food Sci, St Lucia, Qld 4072, AustraliaMiaomiao Cheng - La Trobe UniversityCaixian Tang - La Trobe UniversityDavid J. Paterson - Australian SynchrotronEnzo Lombi - University of South AustraliaWei Hong Wang - University of South AustraliaKirk G. Scheckel - Environmental Protection AgencyPeter M. Kopittke - The University of Queensland
- Publication Details
- Plant physiology (Bethesda), Vol.169(3), pp.2006-2020
- Publisher
- Oxford University Press on behalf of American Society of Plant Biologists.
- Number of pages
- 15
- Grant note
- LP100100800 / Linkage Project; Australian Research Council MRCAT member institutions DE-AC02-06CH11357 / DOE Office of Science by Argonne National Laboratory; United States Department of Energy (DOE) University of Queensland U.S. Department of Energy (DOE); United States Department of Energy (DOE) FT100100337; FT120100277 / Australian Research Council AS153/XFM/8467 / Australian Synchrotron, Victoria, Australia
- Identifiers
- 991005620339007891
- Copyright
- © The Author(s) 2015.
- Murdoch Affiliation
- Centre for Sustainable Farming Systems
- Language
- English
- Resource Type
- Journal article
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