Synovium-Synovial fluid axis in osteoarthritis pathology: A key regulator of the cartilage degradation process

D. Ingale; P. Kulkarni; A. Electricwala; A. Moghe; S. Kamyab; S. Jagtap; A. Martson; S. Kõks; A. Harsulkar

doi:10.3390/genes12070989

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Synovium-Synovial fluid axis in osteoarthritis pathology: A key regulator of the cartilage degradation process

Journal article

Open access

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Synovium-Synovial fluid axis in osteoarthritis pathology: A key regulator of the cartilage degradation process

D. Ingale, P. Kulkarni, A. Electricwala, A. Moghe, S. Kamyab, S. Jagtap, A. Martson, S. Kõks and A. Harsulkar

Genes, Vol.12(7), Art. 989

2021

DOI: https://doi.org/10.3390/genes12070989

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Abstract

Failure of conventional anti-inflammatory therapies in osteoarthritis (OA) underlines the insufficient knowledge about inflammatory mechanisms, patterns and their relationship with cartilage degradation. Considering non-linear nature of cartilage loss in OA, a better understanding of inflammatory milieu and MMP status at different stages of OA is required to design early-stage therapies or personalized disease management. For this, an investigation based on a synovium-synovial fluid (SF) axis was planned to study OA associated changes in synovium and SF along the progressive grades of OA. Gene expressions in synovial-biopsies from different grades OA patients (N = 26) revealed a peak of IL-1β, IL-15, PGE2 and NGF in early OA (Kellgren–Lawrence (KL) grade-I and II); the highest MMP levels were found in advanced stages (KL grade-III and IV). MMPs (MMP-1, 13, 2 and 9) abundance and FALGPA activity estimated in forty SFs of progressive grades showed the maximum protein levels and activity in KL grade-II and III. In an SF challenge test, SW982 and THP1 cells were treated with progressive grade SFs to study the dynamics of MMPs modulation in inflammatory microenvironment; the test yielded a result pattern, which matched with FALGPA and the protein-levels estimation. Inflammatory mediators in SFs served as steering factor for MMP up-regulation. A correlation-matrix of IL-1β and MMPs revealed expressional negative correlation.

Details

Title: Synovium-Synovial fluid axis in osteoarthritis pathology: A key regulator of the cartilage degradation process
Authors/Creators: D. Ingale (Author/Creator) - Bharati Vidyapeeth Deemed University
P. Kulkarni (Author/Creator) - University of Tartu
A. Electricwala (Author/Creator) - Electricwala Hospital, A 4/1, Pleasant Park, Fatima Nagar, Wanowrie, Pune 411013, India.
A. Moghe (Author/Creator) - Bharati Vidyapeeth Deemed University
S. Kamyab (Author/Creator) - Bharati Vidyapeeth Deemed University
S. Jagtap (Author/Creator) - Bharati Vidyapeeth Deemed University
A. Martson (Author/Creator) - Tartu University Hospital
S. Kõks (Author/Creator) - Murdoch University
A. Harsulkar (Author/Creator) - Bharati Vidyapeeth Deemed University
Publication Details: Genes, Vol.12(7), Art. 989
Publisher: MDPI
Identifiers: 991005542382007891
Murdoch Affiliation: Centre for Molecular Medicine and Innovative Therapeutics
Language: English
Resource Type: Journal article

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Collaboration types: Domestic collaboration; International collaboration
Citation topics: 1 Clinical & Life Sciences; 1.34 Orthopedics; 1.34.255 Osteoarthritis
Web Of Science research areas: Genetics & Heredity
ESI research areas: Molecular Biology & Genetics