Journal article
Synthesis of DNA oligonucleotides containing C5-ethynylbenzenesulfonamide-modified nucleotides (EBNA) by polymerases towards the construction of base functionalized nucleic acids
Bioorganic & Medicinal Chemistry Letters, Vol.23(3), pp.761-763
2013
Abstract
C5-Ethynylbenzenesulfonamide-modified nucleotide (EBNA) was investigated as substrate of various DNA polymerases. The experiments revealed that KOD, Phusion and Klenow DNA polymerases successfully accepted EBNA-T nucleotide as a substrate and yielded the fully extended DNA. KOD DNA polymerase was found to be the most efficient enzyme to furnish EBNA-T containing DNA in good yields. Phusion DNA polymerase efficiently amplified the template containing EBNA-T nucleotides by PCR.
Details
- Title
- Synthesis of DNA oligonucleotides containing C5-ethynylbenzenesulfonamide-modified nucleotides (EBNA) by polymerases towards the construction of base functionalized nucleic acids
- Authors/Creators
- A. Goubet (Author/Creator)A. Chardon (Author/Creator)P. Kumar (Author/Creator)P.K. Sharma (Author/Creator)R.N. Veedu (Author/Creator)
- Publication Details
- Bioorganic & Medicinal Chemistry Letters, Vol.23(3), pp.761-763
- Publisher
- Elsevier BV
- Identifiers
- 991005540616907891
- Copyright
- © 2012 Elsevier Ltd.
- Murdoch Affiliation
- Murdoch University
- Language
- English
- Resource Type
- Journal article
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