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Journal article
Systemic perturbations in amino acids/amino acid derivatives and tryptophan pathway metabolites associated with murine influenza A virus infection
Virology journal, Vol.20(1), 270
2023
Abstract
Background
Influenza A virus (IAV) is the only influenza virus causing flu pandemics (i.e., global epidemics of flu disease). Influenza (the flu) is a highly contagious disease that can be deadly, especially in high-risk groups. Worldwide, these annual epidemics are estimated to result in about 3 to 5 million cases of severe illness and in about 290,000 to 650,000 respiratory deaths. We intend to reveal the effect of IAV infection on the host′s metabolism, immune response, and neurotoxicity by using a mouse IAV infection model.
Methods
51 metabolites of murine blood plasma (33 amino acids/amino acid derivatives (AADs) and 18 metabolites of the tryptophan pathway) were analyzed by using Ultra-High-Performance Liquid Chromatography-Mass Spectrometry with Electrospray Ionization at the acute (7 days post-infection (dpi)), resolution (14 dpi), and recovery (21 dpi) stages of the virus infection in comparison with controls.
Results
Among the 33 biogenic amino acids/AADs, the levels of five amino acids/AADs (1-methylhistidine, 5-oxoproline, α-aminobutyric acid, glutamine, and taurine) increased by 7 dpi, whereas the levels of ten amino acids/AADs (4-hydroxyproline, alanine, arginine, asparagine, cysteine, citrulline, glycine, methionine, proline, and tyrosine) decreased. By 14 dpi, the levels of one AAD (3-methylhistidine) increased, whereas the levels of five amino acids/AADs (α-aminobutyric acid, aminoadipic acid, methionine, threonine, valine) decreased. Among the 18 metabolites from the tryptophan pathway, the levels of kynurenine, quinolinic acid, hydroxykynurenine increased by 7 dpi, whereas the levels of indole-3-acetic acid and nicotinamide riboside decreased.
Conclusions
Our data may facilitate understanding the molecular mechanisms of host responses to IAV infection and provide a basis for discovering potential new mechanistic, diagnostic, and prognostic biomarkers and therapeutic targets for IAV infection.
Details
- Title
- Systemic perturbations in amino acids/amino acid derivatives and tryptophan pathway metabolites associated with murine influenza A virus infection
- Authors/Creators
- Huda A M Al-Shalan - Murdoch UniversityLu ZhouZhifan DongPenghao WangPhilip K Nicholls - Murdoch University, School of Medical, Molecular and Forensic SciencesBerin Boughton - Murdoch University, Centre for Computational and Systems MedicinePhilip A StumblesWayne K GreeneBin Ma
- Publication Details
- Virology journal, Vol.20(1), 270
- Publisher
- BioMed Central Ltd unless otherwise stated. Part of Springer Nature
- Identifiers
- 991005617465007891
- Copyright
- © 2023 The Author(s).
- Murdoch Affiliation
- Australian National Phenome Centre; Centre for Computational and Systems Medicine; Centre for Crop and Food Innovation
- Language
- English
- Resource Type
- Journal article
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