Taenia hydatigena: Isolation of mitochondrial DNA, molecular cloning, and physical mitochondrial genome mapping

K.W. Yap; R.C.A. Thompson; J.I. Rood; I.D. Pawlowski

doi:10.1016/0014-4894(87)90175-5

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Taenia hydatigena: Isolation of mitochondrial DNA, molecular cloning, and physical mitochondrial genome mapping

Journal article

Peer reviewed

Taenia hydatigena: Isolation of mitochondrial DNA, molecular cloning, and physical mitochondrial genome mapping

K.W. Yap, R.C.A. Thompson, J.I. Rood and I.D. Pawlowski

Experimental Parasitology, Vol.63(3), pp.288-294

1987

DOI: https://doi.org/10.1016/0014-4894(87)90175-5

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Abstract

Mitochondrial DNA was isolated from Taenia hydatigena, T. crassiceps, and Echinococcus granulosus using a cetyltrimethylammonium bromide precipitation technique. The technique is simple, rapid, reproducible, and does not require extensive high speed ultracentrifugation. The advantage of using mitochondrial DNA from taeniid cestodes for comparative restriction analysis was demonstrated. Mitochondrial DNA of T. hydatigena was isolated as covalently closed circular molecules. These were linearized by single digestion with BamHI and the molecular weight was estimated from the linear form of 17.6 kb. The mitochondrial DNA of T. hydatigena is therefore similar in size and structure to that of many other animal species. The entire mitochondrial genome was cloned into pBR322 in Escherichia coli and a restriction map of the recombinant molecule was constructed. The potential of using the cloned mitochondrial genome as a probe in speciation studies as well as for providing functional information on the role of the cestode mitochondrion is discussed.

Details

Title: Taenia hydatigena: Isolation of mitochondrial DNA, molecular cloning, and physical mitochondrial genome mapping
Authors/Creators: K.W. Yap (Author/Creator)
R.C.A. Thompson (Author/Creator)
J.I. Rood (Author/Creator)
I.D. Pawlowski (Author/Creator)
Publication Details: Experimental Parasitology, Vol.63(3), pp.288-294
Publisher: Academic Press
Identifiers: 991005541751607891
Copyright: © 1987 Published by Elsevier Inc.
Murdoch Affiliation: School of Veterinary Studies
Language: English
Resource Type: Journal article

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Citation topics: 1 Clinical & Life Sciences; 1.163 Parasitology - General; 1.163.1106 Echinococcosis
Web Of Science research areas: Parasitology
ESI research areas: Microbiology