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Published (Version of Record)CC BY-NC-ND V4.0, Open Access
Journal article
Targeted molecular screening for protozoan parasites in wild platypuses (Ornithorhynchus anatinus) and associated risk factors for infection
International journal for parasitology. Parasites and wildlife, Vol.28, 101172
2025
Abstract
The platypus (Ornithorhynchus anatinus) is a unique monotreme endemic to eastern Australia, yet little is known about the prevalence and impact of protozoan infections in wild populations. This study investigated the occurrence of protozoan parasites and associated health parameters in wild platypuses from New South Wales (NSW), Australia, with a focus on seasonal variation and demographic risk factors. A total of 90 sampling events (79 individuals and 11 recaptures) yielded 72 blood and 81 faecal samples for parasitological screening through targeted molecular techniques. Theileria ornithorhynchi was detected in 100 % of blood samples (95 % CI: 95.01–100), confirming its widespread distribution and high prevalence in NSW platypuses. Trypanosoma spp. were identified in 40.3 % of blood samples (95 % CI: 28.88–52.50), and sequencing confirmed the presence of Trypanosoma binneyi, reported here for the first time in NSW. Toxoplasma gondii was detected in one sub-adult male (1.39 %, 95 % CI: 0.04–7.50). No blood samples tested positive for Plasmodium spp. (0 %, 95 % CI: 0–4.99). In addition, all faecal samples tested negative for Cryptosporidium spp. and Giardia sp. (0 %, 95 % CI: 0–4.45). Boosted Regression Tree (BRT) analysis identified season as the primary risk factor for Tr. binneyi infection (RI 89.5 %), with higher prevalence in summer. Males were also at increased risk (RI 10.5 %), while age class and location (river catchment) had no measurable influence. Season also significantly affected physiological parameters, including tail volume index (TVI) and packed cell volume (PCV). These findings expand the known distribution of protozoan parasites and provide new insights into the seasonal dynamics of parasitic infections in wild platypuses. This study contributes to the understanding of platypus health ecology and highlights the importance of long-term, seasonally informed monitoring programs.
Details
- Title
- Targeted molecular screening for protozoan parasites in wild platypuses (Ornithorhynchus anatinus) and associated risk factors for infection
- Authors/Creators
- Renata Fernandes-Santos - School of Veterinary Medicine, College of Environmental and Life Sciences, Murdoch University, Murdoch, WA, 6150, AustraliaJessica Whinfield - School of Veterinary Medicine, College of Environmental and Life Sciences, Murdoch University, Murdoch, WA, 6150, AustraliaMariel Fulham - Macquarie UniversityGilad Bino - UNSW SydneyTahneal Hawke - Taronga Conservation Society AustraliaRebecca Vaughan-Higgins - Murdoch UniversityLarry Vogelnest - Taronga Conservation Society AustraliaKristin S. Warren - Murdoch UniversityMichelle Power - Macquarie University
- Publication Details
- International journal for parasitology. Parasites and wildlife, Vol.28, 101172
- Publisher
- Elsevier Ltd.
- Number of pages
- 10
- Identifiers
- 991005838666807891
- Copyright
- © 2025 The Authors
- Murdoch Affiliation
- Centre for Terrestrial Ecosystem Science and Sustainability; School of Veterinary Medicine
- Language
- English
- Resource Type
- Journal article
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- Collaboration types
- Domestic collaboration
- Citation topics
- 1 Clinical & Life Sciences
- 1.246 Diarrheal Diseases
- 1.246.985 Cryptosporidium
- Web Of Science research areas
- Ecology
- Parasitology
- ESI research areas
- Plant & Animal Science