Temporal tracking of cysteine 34 oxidation of plasma albumin as a biomarker of muscle damage following a bout of eccentric exercise

Christopher James; Cory W Dugan; Corrin Boyd; Paul A Fournier; Peter G Arthur

doi:10.1007/s00421-024-05488-1

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Temporal tracking of cysteine 34 oxidation of plasma albumin as a biomarker of muscle damage following a bout of eccentric exercise

Journal article

Open access

Peer reviewed

Temporal tracking of cysteine 34 oxidation of plasma albumin as a biomarker of muscle damage following a bout of eccentric exercise

Christopher James, Cory W Dugan, Corrin Boyd, Paul A Fournier and Peter G Arthur

European journal of applied physiology, Vol.124(9), pp.2639-2650

2024

DOI: https://doi.org/10.1007/s00421-024-05488-1

PMID: 38627299

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Abstract

Thiol-oxidised albumin

Oxidative stress

Exercise-induced muscle damage

Eccentric exercise

Dried blood spot

Exercise-induced muscle damage (EIMD) results in the generation of reactive oxygen species (ROS), but little is known about the temporal profile of change in ROS post-EIMD and how ROS levels relate to the onset of and recovery from EIMD. Our primary aim was to examine the effect of EIMD on the pattern of change in the blood level of thiol-oxidised albumin, a marker of oxidative stress. Seven male participants were subjected on separate days to eccentric muscle contraction to cause EIMD or a no-exercise condition. After each session, the participants collected daily dried blood spots to measure thiol-oxidised albumin and returned to the laboratory every 2 days for the assessment of indirect markers of EIMD, namely maximal voluntary contraction (MVC), delayed onset muscle soreness (DOMS), creatine kinase (CK), and myoglobin. Eccentric exercise resulted in a significant decrease in MVC and increase in DOMS, CK, myoglobin, and thiol-oxidised albumin with the latter reaching above baseline level within 24-48 h post-exercise. All the markers of EIMD returned to baseline level within 6 days post-exercise, but not the level of thiol-oxidised albumin which remained elevated for 10 days after exercise. There was a moderate correlation between changes in thiol-oxidised albumin and DOMS, but no significant relationship between any other markers of muscle damage. The levels of thiol-oxidised albumin increase in response to EIMD and remain elevated for several days post-exercise. The temporal pattern of change in the level of thiol-oxidised albumin suggests that this may be a useful biomarker of muscle repair post-EIMD.

Details

Title: Temporal tracking of cysteine 34 oxidation of plasma albumin as a biomarker of muscle damage following a bout of eccentric exercise
Authors/Creators: Christopher James - The University of Western Australia
Cory W Dugan - The University of Western Australia
Corrin Boyd - School of Veterinary Medicine, Murdoch University, Murdoch, WA, Australia
Paul A Fournier - The University of Western Australia
Peter G Arthur - The University of Western Australia
Publication Details: European journal of applied physiology, Vol.124(9), pp.2639-2650
Publisher: Springer-Verlag GmbH Germany, part of Springer Nature
Identifiers: 991005656565607891
Murdoch Affiliation: School of Veterinary Medicine
Language: English
Resource Type: Journal article

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Collaboration types: Domestic collaboration
Citation topics: 1 Clinical & Life Sciences; 1.172 Sports Science; 1.172.1542 Exercise Immunology
Web Of Science research areas: Physiology; Sport Sciences
ESI research areas: Clinical Medicine