Journal article
Testin, a novel binding partner of the calcium-sensing receptor, enhances receptor-mediated Rho-kinase signalling
Biochemical and Biophysical Research Communications, Vol.412(4), pp.584-589
2011
Abstract
The calcium-sensing receptor (CaR) plays an integral role in calcium homeostasis and the regulation of other cellular functions including cell proliferation and cytoskeletal organisation. The multifunctional nature of the CaR is manifested through ligand-dependent stimulation of different signalling pathways that are also regulated by partner binding proteins. Following a yeast two-hybrid library screen using the intracellular tail of the CaR as bait, we identified several novel binding partners including the focal adhesion protein, testin. Testin has not previously been shown to interact with cell surface receptors. The sites of interaction between the CaR and testin were mapped to the membrane proximal region of the receptor tail and the second zinc-finger of LIM domain 1 of testin, the integrity of which was found to be critical for the CaR-testin interaction. The CaR-testin association was confirmed in HEK293 cells by coimmunoprecipitation and confocal microscopy studies. Ectopic expression of testin in HEK293 cells stably expressing the CaR enhanced CaR-stimulated Rho activity but had no effect on CaR-stimulated ERK signalling. These results suggest an interplay between the CaR and testin in the regulation of CaR-mediated Rho signalling with possible effects on the cytoskeleton.
Details
- Title
- Testin, a novel binding partner of the calcium-sensing receptor, enhances receptor-mediated Rho-kinase signalling
- Authors/Creators
- A.L. Magno (Author/Creator) - The University of Western AustraliaE. Ingley (Author/Creator) - The University of Western AustraliaS.J. Brown (Author/Creator) - Sir Charles Gairdner HospitalA.D. Conigrave (Author/Creator) - The University of SydneyT. Ratajczak (Author/Creator) - UWA Centre for Medical ResearchB.K. Ward (Author/Creator) - Sir Charles Gairdner Hospital
- Publication Details
- Biochemical and Biophysical Research Communications, Vol.412(4), pp.584-589
- Publisher
- Academic Press
- Identifiers
- 991005539579307891
- Copyright
- © 2011 Elsevier Inc.
- Murdoch Affiliation
- Murdoch University
- Language
- English
- Resource Type
- Journal article
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