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Journal article
The Essentials of PgPG1, a Polygalacturonase-Encoding Gene for the Invasion of Pyrenophora graminea to Hordeum vulgare
International journal of molecular sciences, Vol.26(6), 2401
2025
PMID: 40141049
Abstract
Barley leaf stripe, caused by Pyrenophora graminea, significantly reduces yield. Polygalacturonase, a key fungal pectinase, facilitates cell wall degradation for nutrition acquisition and colonization. To determine whether P. graminea contains polygalacturonase (PgPG)-encoding genes and their role in pathogenicity, four PgPG genes (PgPG1–PgPG4) were identified in the P. graminea genome. Quantitative RT-PCR revealed that PgPG1 had the highest inducible expression during barley infection, suggesting its critical vital role in pathogenesis. PgPG1 was silenced and overexpressed in P. graminea QWC (wild-type) using CaCl2-PEG4000-mediated protoplast transformation. The PgPG1 RNAi mutants exhibited slower growth, while overexpression mutants grew faster. Relative to the wild-type, the disease incidence of Alexis, a highly susceptible barley variety, decreased by 62.94%, 42.19%, 45.74%, and 40.67% for RNAi mutants, and increased by 12.73%, 12.10%, 12.63%, and 10.31% for overexpression mutants. Pathogenicity analysis showed decreased disease incidence with PgPG1 RNAi mutants and increased severity with overexpression mutants. Trypan blue staining and polygalacturonase activity assays confirmed that overexpression mutants caused more severe damage compared to wild-type and RNAi mutants. These findings indicate that PgPG1 plays a vital role in the pathogenicity of P. graminea in barley and has great potential as a pathogen target gene to develop a durable resistance variety to P. graminea.
Details
- Title
- The Essentials of PgPG1, a Polygalacturonase-Encoding Gene for the Invasion of Pyrenophora graminea to Hordeum vulgare
- Authors/Creators
- Erjing Si - Gansu Agricultural UniversityMing Guo - Gansu Agricultural UniversityHaiying Liu - Gansu Agricultural UniversityChengdao Li - Murdoch University, Centre for Crop and Food InnovationJuncheng Wang - Gansu Agricultural UniversityLirong Yao - Gansu Agricultural UniversityYaxiong Meng - Gansu Agricultural UniversityXiaole Ma - Gansu Agricultural UniversityBaochun Li - Gansu Agricultural UniversityKe Yang - Gansu Agricultural UniversityXunwu Shang - State Key Laboratory of Aridland Crop Science, Gansu Key Laboratory of Crop Improvement and Germplasm Enhancement, Lanzhou 730070, ChinaHuajun Wang - Gansu Agricultural University
- Publication Details
- International journal of molecular sciences, Vol.26(6), 2401
- Publisher
- MDPI; BASEL
- Number of pages
- 16
- Grant note
- GSCS-2018-6 / Gansu Provincial Key Laboratory of Aridland Crop Science, Gansu Agricultural University CARS-05-03B-03 / The China Agriculture Research System of MOF and MARA 2021 CYZC-12 / Industrial Support Project of Gansu Provincial Education Department 20JR5RA010 / Gansu Province Youth Science and Technology Fund 22CX8NA047 / Gannan Technology Plan Project 2021A-055 / Innovation Fund project of Education Department of Gansu Province 32160496 / National Natural Science Foundation of China
- Identifiers
- 991005753924307891
- Copyright
- © 2025 by the authors.
- Murdoch Affiliation
- Western Barley Genetics Alliance
- Language
- English
- Resource Type
- Journal article
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- Collaboration types
- Domestic collaboration
- International collaboration
- Citation topics
- 3 Agriculture, Environment & Ecology
- 3.4 Crop Science
- 3.4.1010 Plant Cell Walls
- Web Of Science research areas
- Biochemistry & Molecular Biology
- Chemistry, Multidisciplinary
- ESI research areas
- Chemistry