Journal article
The HKT transporter HvHKT1;5 negatively regulates salt tolerance
Plant Physiology, Vol.182(1), pp.584-596
2019
Abstract
Maintaining low intracellular Na+ concentrations is an essential physiological strategy in salt stress tolerance in most cereal crops. Here, we characterized a member of the high-affinity K+ transporter (HKT) family in barley (Hordeum vulgare), HvHKT1;5, which negatively regulates salt tolerance and has different functions from its homology in other cereal crops. HvHKT1;5 encodes a plasma membrane protein localized to root stele cells, particularly in xylem parenchyma cells adjacent to the xylem vessels. Its expression was highly induced by salt stress. Heterogenous expression of HvHKT1;5 in Xenopus laevis oocytes showed that HvHKT1;5 was permeable to Na+, but not to K+, although its Na+ transport activity was inhibited by external K+. HvHKT1;5 knockdown barley lines showed improved salt tolerance, a dramatic decrease in Na+ translocation from roots to shoots, and increases in K+/Na+ when compared with wild-type plants under salt stress. The negative regulation of HvHKT1;5 in salt tolerance distinguishes it from other HKT1;5 members, indicating that barley has a distinct Na+ transport system. These findings provide a deeper understanding of the functions of HKT family members and the regulation of HvHKT1;5 in improving salt tolerance of barley.
Details
- Title
- The HKT transporter HvHKT1;5 negatively regulates salt tolerance
- Authors/Creators
- L. Huang (Author/Creator) - Zhejiang UniversityL. Kuang (Author/Creator) - Zhejiang UniversityL. Wu (Author/Creator) - Zhejiang UniversityQ. Shen (Author/Creator) - Zhejiang UniversityY. Han (Author/Creator) - Zhejiang UniversityL. Jiang (Author/Creator) - Zhejiang UniversityD. Wu (Author/Creator) - Zhejiang UniversityG. Zhang (Author/Creator) - Zhejiang University
- Publication Details
- Plant Physiology, Vol.182(1), pp.584-596
- Identifiers
- 991005544676907891
- Copyright
- © 2020 American Society of Plant Biologists
- Murdoch Affiliation
- Murdoch University
- Language
- English
- Resource Type
- Journal article
UN Sustainable Development Goals (SDGs)
This output has contributed to the advancement of the following goals:
Source: InCites
Metrics
85 Record Views
InCites Highlights
These are selected metrics from InCites Benchmarking & Analytics tool, related to this output
- Citation topics
- 3 Agriculture, Environment & Ecology
- 3.4 Crop Science
- 3.4.49 Plant Stress Responses
- Web Of Science research areas
- Plant Sciences
- ESI research areas
- Plant & Animal Science