Logo image
Back
The Human Transcription Enhancer Factor-1, TEF-1, Can Substitute for Drosophila scalloped during Wingblade Development
Journal article   Peer reviewed

The Human Transcription Enhancer Factor-1, TEF-1, Can Substitute for Drosophila scalloped during Wingblade Development

N. Deshpande, A. Chopra, A. Rangarajan, L.S. Shashidhara, V. Rodrigues and S. Krishna
Journal of Biological Chemistry, Vol.272(16), pp.10664-10668
1997
DOI: https://doi.org/10.1074/jbc.272.16.10664
url
Link to Published Version *Subscription may be requiredView

Abstract

The human transcription enhancer factor-1 (TEF-1) belongs to a family of evolutionarily conserved proteins that have a DNA binding TEA domain. TEF-1 shares a 98% homology with Drosophila scalloped (sd) in the DNA binding domain and a 50% similarity in the activation domain. We have expressed human TEF-1 in Drosophila under the hsp-70 promoter and find that it can substitute for Sd function. The transformants rescue the wingblade defects as well as the lethality of loss-of-function alleles. Observation of reporter activity in the imaginal wing discs of the enhancer-trap alleles suggests that TEF-1 is capable of promoting sd gene regulation. The functional capability of the TEF-1 product was assessed by comparing the extent of rescue by heat shock (hs)-TEF-1 with that of hs-sd. The finding that TEF-1 can function in vivo during wingblade development offers a potent genetic system for the analysis of its function and in the identification of the molecular partners of TEF-1.

Details

Metrics

19 Record Views
Logo image