The Olive Biophenols Oleuropein and Hydroxytyrosol Selectively Reduce Proliferation, Influence the Cell Cycle, and Induce Apoptosis in Pancreatic Cancer Cells

Chloe D Goldsmith; Danielle R Bond; Helen Jankowski; Judith Weidenhofer; Costas E Stathopoulos; Paul D Roach; Christopher J Scarlett

doi:10.3390/ijms19071937

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The Olive Biophenols Oleuropein and Hydroxytyrosol Selectively Reduce Proliferation, Influence the Cell Cycle, and Induce Apoptosis in Pancreatic Cancer Cells

Journal article

Open access

Peer reviewed

The Olive Biophenols Oleuropein and Hydroxytyrosol Selectively Reduce Proliferation, Influence the Cell Cycle, and Induce Apoptosis in Pancreatic Cancer Cells

Chloe D Goldsmith, Danielle R Bond, Helen Jankowski, Judith Weidenhofer, Costas E Stathopoulos, Paul D Roach and Christopher J Scarlett

International journal of molecular sciences, Vol.19(7), 1937

2018

DOI: https://doi.org/10.3390/ijms19071937

PMID: 30004416

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Abstract

Apoptosis - drug effects

Apoptosis Regulatory Proteins - metabolism

Cell Cycle - drug effects

Cell Line, Tumor

Humans

Iridoids - chemistry

Iridoids - pharmacology

Neoplasm Proteins - metabolism

Olea - chemistry

Pancreatic Neoplasms - drug therapy

Pancreatic Neoplasms - metabolism

Pancreatic Neoplasms - pathology

Phenylethyl Alcohol - analogs & derivatives

Phenylethyl Alcohol - chemistry

Phenylethyl Alcohol - pharmacology

Current chemotherapy drugs for pancreatic cancer only offer an increase in survival of up to six months. Additionally, they are highly toxic to normal tissues, drastically affecting the quality of life of patients. Therefore, the search for novel agents, which induce apoptosis in cancer cells while displaying limited toxicity towards normal cells, is paramount. The olive biophenols, oleuropein, hydroxytyrosol and tyrosol, have displayed cytotoxicity towards cancer cells without affecting non-tumorigenic cells in cancers of the breast and prostate. However, their activity in pancreatic cancer has not been investigated. Therefore, the aim of this study was to determine the anti-pancreatic cancer potential of oleuropein, hydroxytyrosol and tyrosol. Pancreatic cancer cells (MIA PaCa-2, BxPC-3, and CFPAC-1) and non-tumorigenic pancreas cells (HPDE) were treated with oleuropein, hydroxytyrosol and tyrosol to determine their effect on cell viability. Oleuropein displayed selective toxicity towards MIA PaCa-2 cells and hydroxytyrosol towards MIA PaCa-2 and HPDE cells. Subsequent analysis of Bcl-2 family proteins and caspase 3/7 activation determined that oleuropein and hydroxytyrosol induced apoptosis in MIA PaCa-2 cells, while oleuropein displayed a protective effect on HPDE cells. Gene expression analysis revealed putative mechanisms of action, which suggested that c-Jun and c-Fos are involved in oleuropein and hydroxytyrosol induced apoptosis of MIA PaCa-2 cells.

Details

Title: The Olive Biophenols Oleuropein and Hydroxytyrosol Selectively Reduce Proliferation, Influence the Cell Cycle, and Induce Apoptosis in Pancreatic Cancer Cells
Authors/Creators: Chloe D Goldsmith - University of Newcastle Australia
Danielle R Bond - University of Newcastle Australia
Helen Jankowski - Hunter Medical Research Institute
Judith Weidenhofer - Hunter Medical Research Institute
Costas E Stathopoulos - Abertay University
Paul D Roach - Hunter Water
Christopher J Scarlett - University of Newcastle Australia
Publication Details: International journal of molecular sciences, Vol.19(7), 1937
Publisher: MDPI
Identifiers: 991005705533907891
Murdoch Affiliation: Food Futures Institute
Language: English
Resource Type: Journal article

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Collaboration types: Domestic collaboration; International collaboration
Citation topics: 1 Clinical & Life Sciences; 1.68 Lipids; 1.68.621 Virgin Olive Oil
Web Of Science research areas: Biochemistry & Molecular Biology; Chemistry, Multidisciplinary
ESI research areas: Chemistry