The allelic mutation of NBS-LRR gene causes premature senescence in wheat

Lin Qiu; Rongmin Fang; Yong Jia; Hongchun Xiong; Yongdun Xie; Linshu Zhao; G U Jiayu; Shirong Zhao; Yuping Ding; L I Chengdao; Huijun Guo; Luxiang Liu

doi:10.1016/j.plantsci.2025.112395

Back

The allelic mutation of NBS-LRR gene causes premature senescence in wheat

Journal article

Open access

Peer reviewed

The allelic mutation of NBS-LRR gene causes premature senescence in wheat

Lin Qiu, Rongmin Fang, Yong Jia, Hongchun Xiong, Yongdun Xie, Linshu Zhao, G U Jiayu, Shirong Zhao, Yuping Ding, L I Chengdao, …

Plant science (Limerick), Vol.352, 112395

2025

DOI: https://doi.org/10.1016/j.plantsci.2025.112395

PMID: 39842697

Files and links (1)

pdf

Published5.84 MBDownload View

CC BY-NC-ND V4.0, Open Access

Abstract

fine mapping

mutant

NBS-LRR domain

premature senescence

wheat

Premature senescence has a significant impact on the yield and quality of wheat crops. The process is controlled by multiple and intricate genetic pathways and regulatory elements, whereby the discovery of additional mutants provides important insights into the molecular basis of this important trait. Here, we developed a premature senescence wheat mutant je0874, its leaves started to show yellow before heading stage; with plant growth and development, the degree of yellowing worsened rapidly, and chlorophyll content in flag leaf was reduced by 93.8% at 15 days after heading, all other leaves became dryness at the grain filling stage. In the mutant, the reactive oxygen species (ROS) and its metabolites increased up to 34.8%~47.3%, while activities of ROS scavenging enzymes were reduced by 62.7% to 96.7%. Premature senescence resulted in a reduction of thousand grain weight by over 50%. Genetic analysis showed the mutation of senescence was controlled by a single recessive gene, and target gene was finely mapped to a 338kb region of the long arm of chromosome 2D. This region contained a total of 6 annotated genes, while only gene TraesFLD2D01G513900 carried a SNP mutation. The gene contained an NBS-LRR domain, we named it Taps1. Allelic mutants of Taps1 exhibited a lesion mimic phenotype, and the mutant allele resulted in cell death in tobacco, which represent a novel gene controlling wheat senescence. Two haplotypes were identified in 180 accessions, which did not lead to cell death. These results contribute to increase our understanding of the regulation of premature plant senescence.

Details

Title: The allelic mutation of NBS-LRR gene causes premature senescence in wheat
Authors/Creators: Lin Qiu - Chinese Academy of Agricultural Sciences
Rongmin Fang - Institute of Crop Sciences
Yong Jia - Western Crop Genetics Alliance, College of Science, Health, Engineering and Education, Murdoch University, Perth, WA 6150, Australia
Hongchun Xiong - Institute of Crop Sciences, Chinese Academy of Agricultural Sciences / State Key Laboratory of Crop Gene Resources and Breeding / National Center of Space Mutagenesis for Crop Improvement, Beijing, China
Yongdun Xie - Institute of Crop Sciences, Chinese Academy of Agricultural Sciences / State Key Laboratory of Crop Gene Resources and Breeding / National Center of Space Mutagenesis for Crop Improvement, Beijing, China
Linshu Zhao - Institute of Crop Sciences, Chinese Academy of Agricultural Sciences / State Key Laboratory of Crop Gene Resources and Breeding / National Center of Space Mutagenesis for Crop Improvement, Beijing, China
G U Jiayu - Institute of Crop Sciences
Shirong Zhao - Chinese Academy of Agricultural Sciences
Yuping Ding - Chinese Academy of Agricultural Sciences
L I Chengdao - Murdoch University
Huijun Guo - Institute of Crop Sciences, Chinese Academy of Agricultural Sciences / State Key Laboratory of Crop Gene Resources and Breeding / National Center of Space Mutagenesis for Crop Improvement, Beijing, China
Luxiang Liu - Institute of Crop Sciences, Chinese Academy of Agricultural Sciences / State Key Laboratory of Crop Gene Resources and Breeding / National Center of Space Mutagenesis for Crop Improvement, Beijing, China
Publication Details: Plant science (Limerick), Vol.352, 112395
Publisher: Elsevier B.V.; CLARE
Number of pages: 11
Grant note: National Key Research and Development Program: 2022YFD1200700 Crop Varietal Improvement and Insect Pests Control by Nuclear Radiation, China Agriculture Research System of MOF and MARA of P.R. China: CARS-03
This work was supported by the National Key Research and Development Program (Grant No. 2022YFD1200700) , the Crop Varietal Improvement and Insect Pests Control by Nuclear Radiation, China Agriculture Research System of MOF and MARA (CARS-03) of P.R. China. The authors would like to sincerely thank Dr. ZhiyongLiu, Institute of Genetics and Developmental Biology, Chinese Academy of Sciences, for his suggestions on the experiment and providing both binary vector PS1300 and Agrobacterium tumefaciens GV3101.
Identifiers: 991005737345007891
Murdoch Affiliation: Western Crop Genetics Alliance
Language: English
Resource Type: Journal article

UN Sustainable Development Goals (SDGs)

This output has contributed to the advancement of the following goals:

Source: InCites

Metrics

20 File views/ downloads

28 Record Views

3 Times Cited - Web of Science

InCites Highlights

These are selected metrics from InCites Benchmarking & Analytics tool, related to this output

Collaboration types: Domestic collaboration; International collaboration
Citation topics: 3 Agriculture, Environment & Ecology; 3.4 Crop Science; 3.4.419 Jasmonic Acid
Web Of Science research areas: Biochemistry & Molecular Biology; Plant Sciences
ESI research areas: Plant & Animal Science