The challenges of using high-throughput sequencing to track multiple bipartite mycoviruses of wild orchid-fungus partnerships over consecutive years

J.W.L. Ong; H. Li; K. Sivasithamparam; K.W. Dixon; M.G.K. Jones; S.J. Wylie

doi:10.1016/j.virol.2017.07.031

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The challenges of using high-throughput sequencing to track multiple bipartite mycoviruses of wild orchid-fungus partnerships over consecutive years

Journal article

Peer reviewed

The challenges of using high-throughput sequencing to track multiple bipartite mycoviruses of wild orchid-fungus partnerships over consecutive years

J.W.L. Ong, H. Li, K. Sivasithamparam, K.W. Dixon, M.G.K. Jones and S.J. Wylie

Virology, Vol.510, pp.297-304

2017

DOI: https://doi.org/10.1016/j.virol.2017.07.031

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Abstract

The bipartite alpha- and betapartitiviruses are recorded from a wide range of fungi and plants. Using a combination of dsRNA-enrichment, high-throughput shotgun sequencing and informatics, we report the occurrence of multiple new partitiviruses associated with mycorrhizal Ceratobasidium fungi, themselves symbiotically associated with a small wild population of Pterostylis sanguinea orchids in Australia, over two consecutive years. Twenty-one partial or near-complete sequences representing 16 definitive alpha- and betapartitivirus species, and further possible species, were detected from two fungal isolates. The majority of partitiviruses occurred in fungal isolates from both years. Two of the partitiviruses represent phylogenetically divergent forms of Alphapartitivirus, suggesting that they may have evolved under long geographical isolation there. We address the challenge of pairing the two genomic segments of partitiviruses to identify species when multiple partitiviruses co-infect a single host.

Details

Title: The challenges of using high-throughput sequencing to track multiple bipartite mycoviruses of wild orchid-fungus partnerships over consecutive years
Authors/Creators: J.W.L. Ong (Author/Creator)
H. Li (Author/Creator)
K. Sivasithamparam (Author/Creator)
K.W. Dixon (Author/Creator)
M.G.K. Jones (Author/Creator)
S.J. Wylie (Author/Creator)
Publication Details: Virology, Vol.510, pp.297-304
Publisher: Elsevier
Identifiers: 991005545028307891
Copyright: © 2017 Elsevier Inc.
Murdoch Affiliation: School of Veterinary and Life Sciences
Language: English
Resource Type: Journal article

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Collaboration types: Domestic collaboration
Citation topics: 3 Agriculture, Environment & Ecology; 3.267 Virology - Plant; 3.267.356 Plant Virus Interactions
Web Of Science research areas: Virology
ESI research areas: Microbiology