The effect of electron transport (ET) inhibitors and thiabendazole on the fumarate reductase (FR) and succinate dehydrogenase (SDH) of Strongyloides ratti infective (L3) larvae

A. Armson; W.B. Grubb; A.H.W. Mendis

doi:10.1016/0020-7519(94)E0061-Q

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The effect of electron transport (ET) inhibitors and thiabendazole on the fumarate reductase (FR) and succinate dehydrogenase (SDH) of Strongyloides ratti infective (L3) larvae

Journal article

Peer reviewed

The effect of electron transport (ET) inhibitors and thiabendazole on the fumarate reductase (FR) and succinate dehydrogenase (SDH) of Strongyloides ratti infective (L3) larvae

A. Armson, W.B. Grubb and A.H.W. Mendis

International Journal for Parasitology, Vol.25(2), pp.261-263

1995

DOI: https://doi.org/10.1016/0020-7519(94)E0061-Q

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Abstract

The fumarate reductase (FR) and succinate dehydrogenase (SDH) activities of isolated submitochondrial particles (SMPs) prepared from axenised L3 larvae of S. ratti were characterised with respect to their response to a selected range of inhibitors. Rotenone (a specific inhibitor of electron transport Complex I) inhibited the S. ratti FR (EC50 = 3.0 × 10−7m) but not SDH. This strongly suggests that the S. ratti FR is functionally linked with the S. ratti ET-Complex I. 2-Thenoyltrifluoroacetone (TTFA, an inhibitor of ET-Complex II) inhibited FR (EC50 = 2.6 × 10−5m) and SDH (EC50 = 2.8 × 10−5m) with similar effectiveness. Sodium malouate (substrate analogue of succinate) had a greater affinity for SDH (EC50 = 6.8 × 10−4m) than FR (EC50 = 1.9 × 10−2m). Sodium fumarate was ca. 8-fold more effective in inhibiting the S. ratti FR (EC50 = 6.0 × 10−4m) than SDH (EC50 = 4.8 × 10−3m). The S. ratti FR was more sensitive to inhibition by thiabendazole (TBZ; EC50 = 4.6 × 10−4m) than SDH (EC50 > 1.0 × 10−3m), suggesting that one of the sites-of-action of TBZ to be the FR of S. ratti mitochondria. More potent inhibitors of S. ratti FR, if developed, may prove to be effective chemotherapeutic agents in the management of human strongyloidiasis.

Details

Title: The effect of electron transport (ET) inhibitors and thiabendazole on the fumarate reductase (FR) and succinate dehydrogenase (SDH) of Strongyloides ratti infective (L3) larvae
Authors/Creators: A. Armson (Author/Creator)
W.B. Grubb (Author/Creator)
A.H.W. Mendis (Author/Creator)
Publication Details: International Journal for Parasitology, Vol.25(2), pp.261-263
Publisher: Elsevier BV
Identifiers: 991005541296707891
Copyright: 1995 Australian Society for Parasitology
Murdoch Affiliation: Murdoch University
Language: English
Resource Type: Journal article

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Citation topics: 1 Clinical & Life Sciences; 1.163 Parasitology - General; 1.163.1022 Anthelmintic Resistance
Web Of Science research areas: Parasitology
ESI research areas: Microbiology