The effect of histological processing on the form of iron in iron-loaded human tissues

W. Chua‐anusorn; J. Webb; D.J. Macey; P. Pootrakul; T.G. St Pierre

doi:10.1016/S0925-4439(97)00009-4

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The effect of histological processing on the form of iron in iron-loaded human tissues

Journal article

Peer reviewed

The effect of histological processing on the form of iron in iron-loaded human tissues

W. Chua‐anusorn, J. Webb, D.J. Macey, P. Pootrakul and T.G. St Pierre

Biochimica et Biophysica Acta (BBA) - Molecular Basis of Disease, Vol.1360(3), pp.255-261

1997

DOI: https://doi.org/10.1016/S0925-4439(97)00009-4

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Abstract

Iron-loaded human spleen tissue was immersed in neutral buffered formalin over a period of 200 days. Over the first 60 days, iron leached steadily from the tissue until 3% had been lost. Thereafter, no further iron leaching was detected. Comparisons of Mossbauer spectra of freeze-dried tissue and tissue freeze-dried after immersion in formalin for 200 days showed no evidence of chemical transformation of the iron remaining in the tissue. The spectra indicated a difference in the heme-iron to non-heme iron ratio between the two samples probably reflecting inhomogeneity of the ratio throughout the spleen as measured on the centimetre scale. Mossbauer spectra of freeze-dried samples of iron-loaded human liver and pancreas tissue were compared with those for samples from the same patient that had been processed by routine hospital procedures for histology and archival. These spectra showed no evidence for chemical transformation of the iron present in the tissues. These results demonstrate that it is feasible to use archived fixed and embedded human tissue samples for studies aimed at gauging the relative fraction of goethite-like hemosiderin present in the tissue.

Details

Title: The effect of histological processing on the form of iron in iron-loaded human tissues
Authors/Creators: W. Chua‐anusorn (Author/Creator)
J. Webb (Author/Creator) - Murdoch University
D.J. Macey (Author/Creator) - Murdoch University
P. Pootrakul (Author/Creator) - Siriraj Hospital
T.G. St Pierre (Author/Creator)
Publication Details: Biochimica et Biophysica Acta (BBA) - Molecular Basis of Disease, Vol.1360(3), pp.255-261
Publisher: Elsevier BV
Identifiers: 991005543476407891
Copyright: (c) Elsevier BV
Murdoch Affiliation: School of Biological and Environmental Sciences
Language: English
Resource Type: Journal article

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Collaboration types: Domestic collaboration; International collaboration
Citation topics: 1 Clinical & Life Sciences; 1.184 Physiology & Metals; 1.184.573 Iron Metabolism
Web Of Science research areas: Biochemistry & Molecular Biology; Biophysics; Cell Biology
ESI research areas: Clinical Medicine