The effect of prolonged iron loading on the chemical form of iron oxide deposits in rat liver and spleen

W. Chua‐anusorn; J. Webb; D.J. Macey; P. De La Motte Hall; T.G. St Pierre

doi:10.1016/S0925-4439(99)00036-8

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The effect of prolonged iron loading on the chemical form of iron oxide deposits in rat liver and spleen

Journal article

Peer reviewed

The effect of prolonged iron loading on the chemical form of iron oxide deposits in rat liver and spleen

W. Chua‐anusorn, J. Webb, D.J. Macey, P. De La Motte Hall and T.G. St Pierre

Biochimica et Biophysica Acta (BBA) - Molecular Basis of Disease, Vol.1454(2), pp.191-200

1999

DOI: https://doi.org/10.1016/S0925-4439(99)00036-8

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Abstract

Female Porton rats were loaded with iron either by supplementing the diet with 2.5% carbonyl iron for up to 22 months (18 rats) or by regularly injecting rat blood cells intraperitoneally for up to 10 months (eight rats). 57Fe Mossbauer spectroscopy of freeze-dried samples of liver and spleen was used to analyse the chemical forms of iron deposited in these tissues over the period of iron loading. A sextet signal in the Mossbauer spectra was identified as being due to a form of haemosiderin based on the structure of the mineral goethite. The spectral parameters of the sextet signal in the rat tissues indicate that the goethite-like haemosiderin particles are less crystalline than those found in iron-loaded human tissues. For the dietary-iron-loaded rat livers, the fraction (F(s)) of the Mossbauer signal in the form of this sextet was found to increase significantly (from approx 0.04 to 0.09) with the age of the rats (r = 0.77, P < 0.0005). This indicates that the fraction of liver iron in the form of the goethite-like haemosiderin increases with age of the rat and hence with the duration of iron loading. In addition, F(s) for these livers was found to increase significantly with the fraction of iron in non-parenchymal cells as measured by computer-assisted morphometric analysis of histological sections (r = 0.71, P < 0.005).

Details

Title: The effect of prolonged iron loading on the chemical form of iron oxide deposits in rat liver and spleen
Authors/Creators: W. Chua‐anusorn (Author/Creator)
J. Webb (Author/Creator) - Murdoch University
D.J. Macey (Author/Creator) - Murdoch University
P. De La Motte Hall (Author/Creator)
T.G. St Pierre (Author/Creator)
Publication Details: Biochimica et Biophysica Acta (BBA) - Molecular Basis of Disease, Vol.1454(2), pp.191-200
Publisher: Elsevier
Identifiers: 991005540352207891
Copyright: (C) 1999 Elsevier
Murdoch Affiliation: School of Biological Sciences and Biotechnology
Language: English
Resource Type: Journal article

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Collaboration types: Domestic collaboration
Citation topics: 1 Clinical & Life Sciences; 1.184 Physiology & Metals; 1.184.573 Iron Metabolism
Web Of Science research areas: Biochemistry & Molecular Biology; Biophysics; Cell Biology
ESI research areas: Clinical Medicine