Journal article
The effects of in vitro ageing on the composition of the intracellular free amino acid pool of human diploid fibroblasts
Mechanisms of Ageing and Development, Vol.20(4), pp.279-287
1982
Abstract
The composition of the free amino acid pool of the human diploid fibroblast cell line, BCL-D1, was analysed by automated ion-exchange chromatography throughout the life-span of the culture. Significant age-related changes, independent of growth effects, were found with six essential amino acids: a decrease in tyrosine, phenylalanine, leucine, isoleucine and valine, and an increase in methionine. The nature of the changes were suggestive of a decline in the operation of the L-system of amino acid transport with in vitro age. However, it is also possible that age-related changes in the proportions of tRNA iso-acceptors and/or the availability and accuracy of tRNA synthetases were involved.
Details
- Title
- The effects of in vitro ageing on the composition of the intracellular free amino acid pool of human diploid fibroblasts
- Authors/Creators
- Y. Sambuy (Author/Creator)A.H. Bittles (Author/Creator)
- Publication Details
- Mechanisms of Ageing and Development, Vol.20(4), pp.279-287
- Publisher
- Elsevier
- Identifiers
- 991005542502807891
- Murdoch Affiliation
- Murdoch University
- Language
- English
- Resource Type
- Journal article
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