Journal article
The efficacy of three double-microencapsulation methods for preservation of probiotic bacteria
Scientific Reports, Vol.11(1), Art. 13753
2021
Abstract
Lactic acid bacteria (LAB) are used as a probiotic alternative to antibiotics in livestock production. Microencapsulation technology is widely used for probiotic preservation. A variety of microencapsulation protocols have been proposed and compared based on chemicals and mechanical procedures. This study aimed to develop a double-encapsulated coating from alginate (1.5%) and chitosan (0.5%) by extrusion, emulsion, and spray drying methods using the LAB strains Lactobacillus plantarum strains 31F, 25F, 22F, Pediococcus pentosaceus 77F, and P. acidilactici 72N, and to monitor the basic probiotic properties of the encapsulated prototypes. The final products from each microencapsulation protocol were analysed for their appearance, probiotic properties and viable cell count. Using the spray drying method, particles smaller than 15 μm in diameter with a regular spherical shape were obtained, whereas the other methods produced larger (1.4–52 mm) and irregularly shaped microcapsules. After storage for 6 months at room temperature, the LAB viability of the spray-dried particles was the highest among the three methods. In all the LAB strains examined, the encapsulated LAB retained their probiotic properties in relation to acid-bile tolerance and antibacterial activity. This study highlights the efficacy of double-coating microencapsulation for preserving LAB properties and survival rate, and demonstrates its potential for probiotic application in livestock farms.
Details
- Title
- The efficacy of three double-microencapsulation methods for preservation of probiotic bacteria
- Authors/Creators
- P. Pupa (Author/Creator) - Chulalongkorn UniversityP. Apiwatsiri (Author/Creator) - Chulalongkorn UniversityW. Sirichokchatchawan (Author/Creator) - Chulalongkorn UniversityN. Pirarat (Author/Creator) - Chulalongkorn UniversityN. Muangsin (Author/Creator) - Chulalongkorn UniversityA.A. Shah (Author/Creator) - Chulalongkorn UniversityN. Prapasarakul (Author/Creator) - Chulalongkorn University
- Publication Details
- Scientific Reports, Vol.11(1), Art. 13753
- Publisher
- Springer Nature
- Identifiers
- 991005541579607891
- Copyright
- © 2021 The Authors.
- Murdoch Affiliation
- Murdoch University
- Language
- English
- Resource Type
- Journal article
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- Citation topics
- 1 Clinical & Life Sciences
- 1.120 Inflammatory Bowel Diseases & Infections
- 1.120.267 Lactic Acid Bacteria
- Web Of Science research areas
- Food Science & Technology
- ESI research areas
- Agricultural Sciences