The evolutionarily diverged single-stranded DNA-binding proteins SSB1/SSB2 differentially affect the replication, recombination and mutation of organellar genomes in Arabidopsis thaliana

Weidong Zhu; Jie Qian; Yingke Hou; Luke R. Tembrock; Liyun Nie; Yi-Feng Hsu; Yong Xiang; Yi Zou; Zhiqiang Wu

doi:10.1016/j.pld.2024.11.001

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The evolutionarily diverged single-stranded DNA-binding proteins SSB1/SSB2 differentially affect the replication, recombination and mutation of organellar genomes in Arabidopsis thaliana

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The evolutionarily diverged single-stranded DNA-binding proteins SSB1/SSB2 differentially affect the replication, recombination and mutation of organellar genomes in Arabidopsis thaliana

Weidong Zhu, Jie Qian, Yingke Hou, Luke R. Tembrock, Liyun Nie, Yi-Feng Hsu, Yong Xiang, Yi Zou and Zhiqiang Wu

Plant diversity, Vol.47(1), pp.127-135

2024

DOI: https://doi.org/10.1016/j.pld.2024.11.001

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Abstract

Mutation

Organellar genomes

Recombination

Replication

SSB

Single-stranded DNA-binding proteins (SSBs) play essential roles in the replication, recombination and repair processes of organellar DNA molecules. In Arabidopsis thaliana, SSBs are encoded by a small family of two genes (SSB1 and SSB2). However, the functional divergence of these two SSB copies in plants remains largely unknown, and detailed studies regarding their roles in the replication and recombination of organellar genomes are still incomplete. In this study, phylogenetic, gene structure and protein motif analyses all suggested that SSB1 and SSB2 probably diverged during the early evolution of seed plants. Based on accurate long-read sequencing results, ssb1 and ssb2 mutants had decreased copy numbers for both mitochondrial DNA (mtDNA) and plastid DNA (ptDNA), accompanied by a slight increase in structural rearrangements mediated by intermediate-sized repeats in mt genome and small-scale variants in both genomes. Our findings provide an important foundation for further investigating the effects of DNA dosage in the regulation of mutation frequencies in plant organellar genomes.

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Title: The evolutionarily diverged single-stranded DNA-binding proteins SSB1/SSB2 differentially affect the replication, recombination and mutation of organellar genomes in Arabidopsis thaliana
Authors/Creators: Weidong Zhu - Agricultural Genomics Institute at Shenzhen
Jie Qian - Southwest University
Yingke Hou - Agricultural Genomics Institute at Shenzhen
Luke R. Tembrock - Colorado State University
Liyun Nie - Agricultural Genomics Institute at Shenzhen
Yi-Feng Hsu - Southwest University
Yong Xiang - Agricultural Genomics Institute at Shenzhen
Yi Zou - Agricultural Genomics Institute at Shenzhen
Zhiqiang Wu - Huazhong Agricultural University
Publication Details: Plant diversity, Vol.47(1), pp.127-135
Publisher: Elsevier B.V. on behalf of KeAi Communications Co., Ltd.
Identifiers: 991005718968607891
Murdoch Affiliation: School of Medical, Molecular and Forensic Sciences
Language: English
Resource Type: Journal article

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