The fhu genes of Rhizobium leguminosarum, specifying siderophore uptake proteins: fhuDCB are adjacent to a pseudogene version of fhuA

J.B. Stevens; R.A. Carter; H. Hussain; K.C. Carson; M.J. Dilworth; A.W.B. Johnston

doi:10.1099/13500872-145-3-593

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The fhu genes of Rhizobium leguminosarum, specifying siderophore uptake proteins: fhuDCB are adjacent to a pseudogene version of fhuA

Journal article

Peer reviewed

The fhu genes of Rhizobium leguminosarum, specifying siderophore uptake proteins: fhuDCB are adjacent to a pseudogene version of fhuA

J.B. Stevens, R.A. Carter, H. Hussain, K.C. Carson, M.J. Dilworth and A.W.B. Johnston

Microbiology, Vol.145(3), pp.593-601

1999

DOI: https://doi.org/10.1099/13500872-145-3-593

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Abstract

A mutant of Rhizobium leguminosarum was isolated which fails to take up the siderophore vicibactin. The mutation is in a homologue of fhuB, which in Escherichia coli specifies an inner-membrane protein of the ferric hydroxamate uptake system. In Rhizobium, fhuB is in an operon fhuDCB, which specifies the cytoplasmic membrane and periplasmic proteins involved in siderophore uptake, fhuDCB mutants make vicibactin when grown in Fe concentrations that inhibit its production in the wild-type. Nodules on peas induced by fhuDCB mutants were apparently normal in N2 fixation. Transcription of an fhuDCB-lacZ fusion was Fe-regulated, being approximately 10-fold higher in Fe-depleted cells. Downstream of fhuB, in the opposite orientation, is a version of fhuA whose homologues in other bacteria specify hydroxamate outer-membrane receptors. This fhuA gene appears to be a pseudogene with stop codons and undetectable expression.

Details

Title: The fhu genes of Rhizobium leguminosarum, specifying siderophore uptake proteins: fhuDCB are adjacent to a pseudogene version of fhuA
Authors/Creators: J.B. Stevens (Author/Creator) - University of East Anglia
R.A. Carter (Author/Creator) - University of East Anglia
H. Hussain (Author/Creator) - University of East Anglia
K.C. Carson (Author/Creator) - Murdoch University
M.J. Dilworth (Author/Creator) - Murdoch University
A.W.B. Johnston (Author/Creator) - University of East Anglia
Publication Details: Microbiology, Vol.145(3), pp.593-601
Publisher: Society for General Microbiology
Identifiers: 991005544541907891
Murdoch Affiliation: Centre for Rhizobium Studies; School of Biological Sciences and Biotechnology
Language: English
Resource Type: Journal article

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