Journal article
The first SSR-based genetic linkage map for cultivated groundnut (Arachis hypogaea L.)
Theoretical and Applied Genetics, Vol.118(4), pp.729-739
2009
Abstract
Molecular markers and genetic linkage maps are pre-requisites for molecular breeding in any crop species. In case of peanut or groundnut (Arachis hypogaea L.), an amphidiploid (4X) species, not a single genetic map is, however, available based on a mapping population derived from cultivated genotypes. In order to develop a genetic linkage map for tetraploid cultivated groundnut, a total of 1,145 microsatellite or simple sequence repeat (SSR) markers available in public domain as well as unpublished markers from several sources were screened on two genotypes, TAG 24 and ICGV 86031 that are parents of a recombinant inbred line mapping population. As a result, 144 (12.6%) polymorphic markers were identified and these amplified a total of 150 loci. A total of 135 SSR loci could be mapped into 22 linkage groups (LGs). While six LGs had only two SSR loci, the other LGs contained 3 (LG_AhXV) to 15 (LG_AhVIII) loci. As the mapping population used for developing the genetic map segregates for drought tolerance traits, phenotyping data obtained for transpiration, transpiration efficiency, specific leaf area and SPAD chlorophyll meter reading (SCMR) for 2 years were analyzed together with genotyping data. Although, 2–5 QTLs for each trait mentioned above were identified, the phenotypic variation explained by these QTLs was in the range of 3.5–14.1%. In addition, alignment of two linkage groups (LGs) (LG_AhIII and LG_AhVI) of the developed genetic map was shown with available genetic maps of AA diploid genome of groundnut and Lotus and Medicago. The present study reports the construction of the first genetic map for cultivated groundnut and demonstrates its utility for molecular mapping of QTLs controlling drought tolerance related traits as well as establishing relationships with diploid AA genome of groundnut and model legume genome species. Therefore, the map should be useful for the community for a variety of applications.
Details
- Title
- The first SSR-based genetic linkage map for cultivated groundnut (Arachis hypogaea L.)
- Authors/Creators
- R.K. Varshney (Author/Creator) - International Crops Research Institute for the Semi-Arid TropicsD.J. Bertioli (Author/Creator) - Universidade Católica de BrasíliaM.C. Moretzsohn (Author/Creator) - Brazilian Agricultural Research CorporationV. Vadez (Author/Creator) - International Crops Research Institute for the Semi-Arid TropicsL. Krishnamurthy (Author/Creator) - International Crops Research Institute for the Semi-Arid TropicsR. Aruna (Author/Creator) - International Crops Research Institute for the Semi-Arid TropicsS.N. Nigam (Author/Creator) - International Crops Research Institute for the Semi-Arid TropicsB.J. Moss (Author/Creator) - International Crops Research Institute for the Semi-Arid TropicsK. Seetha (Author/Creator) - International Crops Research Institute for the Semi-Arid TropicsK. Ravi (Author/Creator) - International Crops Research Institute for the Semi-Arid TropicsG. He (Author/Creator) - Tuskegee UniversityS.J. Knapp (Author/Creator) - University of GeorgiaD.A. Hoisington (Author/Creator) - International Crops Research Institute for the Semi-Arid Tropics
- Publication Details
- Theoretical and Applied Genetics, Vol.118(4), pp.729-739
- Publisher
- Springer Verlag
- Identifiers
- 991005543211907891
- Copyright
- © 2008 The Authors.
- Murdoch Affiliation
- Murdoch University
- Language
- English
- Resource Type
- Journal article
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- Citation topics
- 3 Agriculture, Environment & Ecology
- 3.4 Crop Science
- 3.4.96 QTL
- Web Of Science research areas
- Agronomy
- Genetics & Heredity
- Horticulture
- Plant Sciences
- ESI research areas
- Agricultural Sciences